化-重化策略用于制造具有双相互作用能力的生物相容BSA基纳米颗粒,以实现高效的细胞内传递
Tuba Gök1, Elżbieta Okła2, Inessa Halets-Bui3
1Chemistry Dept. Biochemistry Division, Faculty of Arts and Science, Aksaray University, Aksaray, Turkey.
International journal of biological macromolecules
|December 31, 2025
概括
这项研究提出了一种新的去化辅助方法,用于制造用于药物输送的牛血清白蛋白 (BSA) 纳米颗粒. 这些生物相容的纳米载体有效地封装水友性和疏水性化合物,显示出细胞内输送的前景.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术 纳米技术
- 药物输送系统 药物输送系统
背景情况:
- 开发高效和生物相容的纳米载体对于有效的药物输送至关重要.
- 牛血清白蛋白 (BSA) 是一个有前途的生物聚合物用于纳米粒子制造,由于其生物相容性.
研究的目的:
- 开发一种化辅助的策略,用于制备基于BSA的纳米粒子 (NP),用于封装水友和疏水化合物.
- 描述这些新型NP的结构性质,封装效率和体外性能.
主要方法:
- 控制BSA的变性和重新变性以形成NP.
- 封装模型的水友性 (罗达B) 和疏水性 (ANS) 化合物.
- 使用ATR-FTIR,循环二元化 (CD) 光谱,光分析,细胞毒性检测,流细胞计和共聚焦显微镜进行表征.
主要成果:
- 具有高封装效率的稳定NP (RdB为64.7%,ANS为69.6%) 在尺寸和形态上的最小变化下形成.
- 结构分析证实了复合物的成功结合和BSA在重新化后的部分结构恢复.
- NP在体外具有很高的生物相容性 (>95%的细胞活力) 和高效的细胞内化,而疏水性ANS载荷的NP的吸收率更高.
结论:
- 一种简单而通用的去性化-再性化方法使得基于BSA的纳米载体的制造成为可能.
- 这些NP是生物活性化合物的细胞内传递的一个有希望的平台.
- 该方法为设计用于生物医学应用的功能性蛋白质基纳米载体提供了一个新的策略.
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