诱导阶段分离和从发酵中恢复2,3-butanediol
Ryan J Stoklosa1, Renee J Latona1
1Sustainable Biofuels and Co-Products Research Unit, Eastern Regional Research Center USDA-ARS, Wyndmoor, Pennsylvania 19038, United States.
ACS omega
|January 1, 2026
概括
生物基的2,3-butanediol (2,3-BDO) 生产面临着昂贵的分离挑战. 这项研究优化了使用无机盐和有机溶剂的发酵的回收,达到高达85.5%的产量.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 可持续化学 可持续化学
背景情况:
- 2,3-butanediol (2,3-BDO) 是一种多功能平台化学物质,在制药和可持续航空燃料 (SAF) 中具有应用.
- 目前以石油为基础的生产是不可持续的,而通过细菌发酵的生物基生产面临着由于2,3-BDO的高沸点而面临能源密集的分离挑战.
- 开发具有成本效益和有效的生物基2,3-BDO回收方法对于其商业可行性至关重要.
研究的目的:
- 评估和优化使用诱导相分离从发酵中2,3-BDO的分离和回收.
- 为了研究不同无机盐 (NaCl, (NH4) 2SO4) 和有机溶剂 (乙醇,乙烯酸乙酸) 对2,3-BDO回收的有效性.
- 为了确定最大限度地提高2,3-BDO回收率的最佳条件,使用最小的化学投入.
主要方法:
- 在糖蜜中培养*Paenibacillus polymyxa*以通过发酵产生2,3-BDO.
- 使用不同度和NaCl或 (NH4) 2SO4与乙醇 (EtOH) 和/或乙烯酸 (EtAc) 组合的诱导相分离的应用.
- 在不同的分离条件下,2,3-BDO回收产量的量化.
主要成果:
- *Paenibacillus polymyxa*发酵产生了38.9g/L的2.3-BDO在1.5L尺度上.
- 最高的2,3-BDO回收率为85.5%,使用20% (w/v) (NH4) 2SO4与1:1的EtOH/EtAc.Ac混合物实现.
- 在EtOH中20% (w/v) 的 (NH4) 2SO4获得了75.5%的回收,而在EtAc中60% (w/v) 的NaCl获得了73.4%的回收.
- 与NaCl相比,较低的无机盐负载对 (NH4) 2SO4有效,而不是NaCl.
结论:
- 使用无机盐和有机溶剂诱导相分离是一种有希望的替代能源密集型蒸生物基2,3-BDO回收.
- 最佳的回收可以在低化学负载下实现,特别是使用硫酸与乙醇/乙酸混合物.
- 为了经济的大规模生产,需要进一步增加发酵度,但开发的回收方法显示了可持续的2,3-BDO生产的巨大潜力.
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