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High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

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Updated: Jan 7, 2026

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

The Evolution of Silica Nanoparticle-polyester Coatings on Surfaces Exposed to Sunlight

Published on: October 11, 2016

使用反向液体染色学进行体花素表面特征测定.

Oluwatimilehin O Fadiran¹, J Carson Meredith¹

¹School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, NW, Atlanta, Georgia 30032, United States.

|January 1, 2026

概括

这项研究使用反向液态色谱来分析花粉.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
生物材料是一种生物材料.

背景情况:

花粉埃克辛,由运动花粉素组成,具有独特的化学耐药性.
了解运动花粉素的表面化学是其自然作用和材料应用的关键.

研究的目的:

量化探测体花粉素与有机化合物的表面相互作用.
为了研究脱脂和酸处理的花粉的表面化学成分.

主要方法:

采用反向液态色谱法,使用杂草花粉作为静止阶段.
在不同温度下测量各种化学探针的容量因子.
计算的相互作用热量,以确定表面的特性.

主要成果:

脱脂花粉的相互作用很小,除了与乙醇等小型极性分子的相互作用.
经过酸处理的花粉显示出极性和前极性探针 (酒精,氨基) 的强烈保留.
相互作用的度范围从-9到-41kJ/mol,表明特定的表面相互作用.

结论:

经酸处理的花粉表面是极性的,具有易斯酸特征.

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Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Published on: June 26, 2020

运动花粉素的表面化学可以被修改为量身定制的分子相互作用.

反向液态色谱对于表征花粉表面特性是有效的.