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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

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Updated: Jan 7, 2026

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene

Published on: August 22, 2017

射击对射击采集超快的电子衍射射线.

Rémi Claude¹, Michele Puppin², Bruce Weaver

¹École Polytechnique Fédérale de Lausanne, Laboratory of Ultrafast Microscopy and Electron Scattering (LUMES), IPHYS, Faculty of Basic Sciences, Station 6, CH-1015 Lausanne, Switzerland.

Structural dynamics (Melville, N.Y.)

|January 1, 2026

概括

此摘要是机器生成的。

我们开发了一种新的射击对射击采集方法,用于超快速电子衍射实验. 这种技术显著提高了信号与噪声的比率,改善了散射研究中的数据质量.

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User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy

Published on: July 29, 2021

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Last Updated: Jan 7, 2026

$Measurements of Long-range Electronic Correlations During Femtosecond Diffraction Experiments Performed on Nanocrystals of Buckminsterfullerene$

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Published on: August 22, 2017

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科学领域:

超快电子散射是一种超快的电子散射.
材料科学是一种材料科学.
物理化学物理化学

背景情况:

超快速散射实验需要高的信号噪声比率才能获得准确的数据.
传统的设置通常在数据采集速度和质量方面面临限制.
提高时间解析电子衍射的分辨率对于研究动态过程至关重要.

研究的目的:

为光学-keV电子能量探测器引入一种新的射击对射击采集方法.
在超快速散射实验中增强信号噪声比.
描述新采购计划的噪音性能.

主要方法:

将相锁采集方案集成到传统的超快电子衍射装置中.
在20kHz的高重复率下运行.
在各种配置和现实的场景下全面描述噪声水平.

主要成果:

射击对射击采集方法将信号噪声比提高一个数量级.
对不同的实验配置进行了详细的噪声表征.
该方法在现实的实验场景中显示出强度.

结论:

新的射击对射击采集方法为超快速散射提供了显著的数据质量改善.
这种技术在其他高重复率电子衍射和光谱设置中很容易实现.
增强的信号噪声比率有助于更精确地研究超高速现象.