临床,基因组和功能性特征的抗万科米辛的Enterococci从免疫功能低下的患者:洞察到上皮功能障碍和血液感染
Giuseppe Sangiorgio1, Ilenia Martina Pia Filannino1, Giuseppe Migliorisi2
1Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Frontiers in cellular and infection microbiology
|January 1, 2026
概括
在免疫功能低下的患者中,抗万科米辛耐药性肠球菌 (VRE) 显示出抗微生物耐药性和病毒性. 由于VRE的过氧化产量增加,导致上皮细胞损伤和潜在的转移.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 抗万科胺素的Enterococcus faecium和Enterococcus faecalis (VRE) 是机会性病原体,在免疫功能低下的个体中引起血液感染 (BSI).
- 描述VRE分离物和了解它们的致病机制对于管理高风险患者感染至关重要.
研究的目的:
- 通过包括基因组分析在内的免疫功能低下患者的VRE分离物进行鉴定.
- 研究VRE培养超对Caco-2上皮细胞的影响,重点关注粘附和细胞毒性.
主要方法:
- 对来自意大利两家医院的46个VRE分离物的回顾性研究.
- 使用Caco-2细胞单层进行抗微生物敏感性测试,全基因组测序,以及粘附和细胞毒性的体外分析.
- 测量过氧化 (H2O2) 生产量.
主要成果:
- VRE分离物,主要是E. faecium,表现出多种耐药性决定因素.
- E. faecalis 的分离物体显示出遗传多样性,并携带病毒性基因.
- 血流衍生VRE分离物对Caco-2细胞具有更强的粘附性,它们的超剂降低了上皮细胞活力.
- 在某种VRE分离物的细胞模型中观察到高H2O2产量.
结论:
- VRE分离物表现出抗微生物耐药性和毒性特征的融合.
- 细胞毒性代谢物的紧张依赖的粘附和分泌有助于VRE的病变发生.
- 增加的H2O2产量可能会调解上皮损伤和转位,这表明氧化应激是VRE相关病理的一个因素.
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