益生菌活微针是由细菌间竞争设计的,以加速感染伤口的愈合
Honglei Wang1, Zibei Wu1, Chen Gao2
1Key Laboratory of Tropical Biological Resources of the Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou 570200, China.
Acta pharmaceutica Sinica. B
|January 1, 2026
概括
微针中的工程益生菌通过释放抗生素和杀死病原体来对抗慢性伤口感染. 这种创新的微针疗法显著加快了伤口愈合.
科学领域:
- 生物材料科学 生物材料科学
- 微生物学 微生物学
- 药物运输 药物运输 药物运输
背景情况:
- 益生菌治疗显示出慢性感染伤口管理的潜力,但面临着病原体竞争和生物膜透的挑战.
- 现有的治疗方法难以有效地将抗微生物药物深入感染组织并克服微生物防御.
研究的目的:
- 开发一个多功能微针 (MN) 平台,将工程益生菌与光热和抗生素释放能力相结合,用于增强慢性感染伤口治疗.
- 克服益生菌治疗的局限性,包括在富含病原体的环境中竞争力低下以及生物膜屏障的穿透不足.
主要方法:
- 开发DOX@MPN-PLL@Lr (DMP@Lr) 通过涂层Lactobacillus reuteri (Lr) 与包装多克索鲁比 (DOX) 的聚-l-氨酸修饰金属网络 (MPN).
- 将DMP@Lr纳入与营养丰富的溶解微针 (MNs) 中,以提高性能和益生菌功能.
- 利用MPN多功能以光热辅助杀死细菌和近红外 (NIR) 促进抗生素释放,以减少病原体负担并提高益生菌的竞争力.
- 采用MNs进行物理生物膜破坏和精确地传送到深部感染部位.
主要成果:
- *体外*研究表明,DMP@Lr.的杀菌效率高于99.9% (5.5 log).
- 与空白微针相比,微针的生物膜清除率高出1.4倍.
- *体内*实验显示,单个DMP@Lr MN贴片促进了93.9%的伤口关闭,表明强大的抗菌活性和加速愈合.
结论:
- 开发的基于益生菌的微针平台提供了一种创新和可翻译的方法,可以有效治疗慢性感染的伤口.
- 这种多功能微针系统增强了益生菌的竞争力,克服了生物膜障碍,从而显著改善了伤口愈合.
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