相关实验视频
Updated: Jun 16, 2026
09:37
Bioreactor Assembly for Continuous Culture of Complex Fecal Communities
Published on: April 25, 2025
1.0K
使用微生物群保存套件进行便代谢学标准化:对多基因组学整合的影响
Yu Ra Lee1, Jae-Ho Park1, Hye Hyun Yoo2
1Food Functionality Research Division, Korea Food Research Institute, Wanju-Gun, 55365, Jeollabuk-do, Republic of Korea, kfri.re.kr.
International journal of analytical chemistry
|January 1, 2026
概括
建议使用OMNIgene·GUT套件对便代谢量进行湿体重正常化. 这种方法确保了人类健康和营养研究的可重复结果,改善了多学科研究中的数据质量.
科学领域:
- 人类健康与营养
- 代谢学 代谢学 代谢学
- 多种组合的多种组合.
背景情况:
- 在人类健康和营养方面,综合性omics研究正在增长.
- 对于代谢物质的不稳定便样本的最佳储存和预处理仍然具有挑战性.
研究的目的:
- 系统评估三种规范化方法 (湿量,干量,蛋白质定量) 用于定量便代谢概况.
- 通过使用OMNIgene·GUT套件来评估便代谢物概况的可复制性.
主要方法:
- 评估湿量,干量和蛋白质量定量标准化方法.
- 通过OMNIgene·GUT套件收集的健康人和肥胖患者的新鲜便样本中的41种代谢物的分析.
- 使用变化系数 (CV <30) 评估代谢物可再生性.
主要成果:
- 湿和干重的正常化显示在新鲜样品 (24代谢物,CV<30) 中具有很高的可重复性.
- OMNIgene·GUT套件样本显示,在湿量 (20代谢物,CV < 30) 和蛋白质量定量 (19代谢物,CV < 30) 的正常化下,可再生性得到改善.
- 干燥体重正常化未能满足肥胖患者样本的CV<30标准.
- 没有干燥的直接分析提高了色谱清晰度.
结论:
- 湿重正常化为便代谢物概况提供了一个强大的标准化框架,始终尽量减少变化.
- OMNIgene·GUT套件与广泛的代谢分析兼容,适合多种工作流程.
- 可复制的正常化协议对于临床和营养研究中准确,可比,可扩展的便代谢学至关重要.
相关概念视频
01:08Evolution of Microbial Genome
Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
01:29Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
01:24Microbial Classification System
Classification is the process of organizing organisms into hierarchically inclusive groups based on their phenotypic similarities or evolutionary relationships. A species comprises one or more strains, and closely related species are grouped into genera. Genera are further classified into families, families into orders, orders into classes, and so forth, up to the domain level, which is the broadest taxonomic rank derived from a combination of phenotypic and genotypic data.The nomenclature of...