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说明M-MDSCs的功能异质性,以预测败血症的结果

Guoxing Tang¹, Wenjin Xing¹, Lin Zhu²

¹Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Journal of advanced research

|January 6, 2026

概括

败血症免疫抑制涉及到单细胞髓质衍生抑制细胞 (M-MDSCs). 这项研究将HLA-DRlowCD14+单细胞确定为M-MDSCs,揭示了子集和用于预测败血症结果的新型标记物 (VSIG4).

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
基因组学就是基因组学.

背景情况:

败血症是全球主要的死亡原因.
单细胞在败血症中起着双重作用,导致炎症和免疫抑制.
在败血症中,免疫抑制的单细胞髓质衍生抑制细胞 (M-MDSCs) 的特征尚不清楚.

研究的目的:

为了研究M-MDSCs在败血症中的功能异质性.
确定M-MDSCs的新型标记物及其在败血症预后中的潜力.

主要方法:

评估M-MDSC频率用于败血症预后.
使用单细胞RNA测序 (scRNA-seq) 对来自败血症患者的净化HLA-DRhighCD14+和HLA-DRlowCD14+单细胞进行测序.
分析了基因表达和确定了M-MDSC子集.

主要成果:

使用古典标记物的M-MDSC频率由于异质性而具有有限的预后价值.
scRNA-seq将HLA-DRlowCD14+单细胞确定为M-MDSCs,具有高RETN和低HLA-DPB1表达.
确定了五个M-MDSC子集 (IL1R2,THBS1,S100A,IFN-sti,PPBP),具有不同的功能和发展阶段.
VSIG4被确定为免疫抑制性M-MDSCs (THBS1_M-MDSC) 的新型标记物.
在HLA-DRlowCD14+单细胞上对VSIG4和IL1R2的联合检测显示了预测败血症结果的潜力.

结论:

这项研究阐明了M-MDSC在败血症中的异质性和特征.
确定了M-MDSCs的新型标记物和子集,为败血症预后提供了新的方向.

关键词:

HLA-DR ((低) CD14 ((+) 单细胞异质性异质性异质性在 IL1R2 中, IL1R2 是 IL1R2.在M-MDSCs.在VSIG4中使用VSIG4.

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