BdNOX5通过ROS调节在Bactrocera dorsalis中调节肠道微生物群的稳态
Tian Zeng1, Jin-Hui Wu1, Ting Yang1
1Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou, China.
Pest management science
|January 6, 2026
概括
肠道上皮细胞使用BdNOX5来控制活性氧物种 (ROS) 并保持肠道微生物平衡. BdNOX5与BdDUOX一起工作,突出显示了宿主微生物相互作用中的ROS信号.
科学领域:
- 分子生物学分子生物学
- 微生物学 微生物学
- 昆虫生理学 昆虫生理学
背景情况:
- 反应性氧物种 (ROS) 对肠道微生物平衡至关重要.
- NADPH氧化酶是控制ROS产生的主要来源.
- 除了DUOX之外,NADPH氧化酶在昆虫肠道ROS生成中的作用尚不清楚.
研究的目的:
- 识别和表征新型的NADPH氧化酶基因,这些基因参与了Bactrocera dorsalis.肠道ROS的产生.
- 阐明BdNOX5在调节肠道ROS水平和微生物平衡中的作用.
- 研究不同NADPH氧化酶在维持肠道微生物群平衡中的相互作用.
主要方法:
- 在Bactrocera dorsalis中识别和测序BdNOX5的基因.
- 在成年中肠和前肠组织中分析BdNOX5的表达.
- 功能性研究涉及干扰BdNOX5表达 (例如,敲击).
- 对ROS和H2O2水平的测量.
- 对肠道微生物群结构的分析.
- 外源的H2O2补充实验.
- 在BdNOX5倒置后评估BdDUOX表达.
主要成果:
- 在Bactrocera dorsalis中发现了一种新的NADPH氧化酶基因BdNOX5,在成年中肠和前肠中表达高.
- 与BdNOX5表达的干扰降低了中肠ROS和H2O2水平,破坏了微生物群结构.
- 破坏的微生物群恒温是可逆的外源H2O2补充.
- 抑制BdNOX5导致BdDUOX的补偿上调,恢复微生物平衡.
结论:
- BdNOX5 是一个关键基因,用于维持 Bactrocera dorsalis.在肠道ROS水平.
- BdNOX5与BdDUOX协调,以调节肠道微生物平衡.
- 这些发现为通过ROS信号通路对肠道微生物群的宿主调节提供了新的见解.
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