在Puccinia striiformis中,ABCG转运基因PstABCG2有助于多药性耐药性
Fan Ji1, Xinpei Gao1, Yaning Liu1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production and College of Plant Protection, Northwest A & F University, Yangling 712100, P. R. China.
Journal of agricultural and food chemistry
|January 6, 2026
概括
小麦条纹 (Pst) 对杀菌剂的耐药性威胁到粮食安全. 研究人员确定PstABCG2是赋予多药耐药性 (MDR) 的关键基因,为管理这种破坏性小麦疾病提供了洞察力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 在Puccinia striiformis f. sp. 中出现了三胺抗性. 三 (Pst) 对小麦生产构成重大威胁.
- 了解耐药性对新型杀菌剂 (如flubeneteram) 的分子基础对于有效的疾病管理至关重要.
研究的目的:
- 确定Pst.中的三美和乙烯抗性背后的分子机制.
- 阐明ABC转运基因在杀菌剂耐药性中的作用.
- 调查控制耐药性基因表达的调控网络.
主要方法:
- 转录组分析以确定在杀菌剂压力下差异表达的基因.
- RNA干扰 (RNAi) 和宿主诱导的基因沉默 (HIGS) 来评估基因功能.
- 在Fusarium graminearum中异质表达以确认基因功能.
- 结构和生物物理分析以确定杀剂结合相互作用.
- 基因沉默用于研究转录因子的作用.
主要成果:
- 鉴定出ABC载体基因PstABCG2是Pst.中多药耐药性 (MDR) 的关键决定因素.
- 在真菌杀菌剂压力下,PstABCG2表达显著上调.
- 制PstABCG2增强了真菌对三美和流甲胺的敏感性.
- 在PstABCG2中发生的一种突变 (E1184Y) 影响了triadimefon的结合,但没有影响flubeneteram的结合,这表明了适应性抵抗机制.
- 发现GATA家族转录因子PstGATA可以直接激活PstABCG2的表达.
结论:
- 在Pst.ABCG2中,PstABCG2在赋予Pst.对杀菌剂的耐药性方面发挥着至关重要的作用.
- 这些发现为了解Pst中的MDR以及开发抵抗管理策略提供了分子基础.
- 针对PstABCG2或其监管元素可能是开发新型真菌杀菌剂的一个有希望的方法.
相关概念视频
01:31ABC Transporters: Exporter
6.3K
ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
6.3K
01:06Carrier-Mediated Transport
1.1K
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.1K
01:27ABC Transporters: Importer
3.4K
ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
3.4K
02:56Treatment Resistant Cancers
3.7K
Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.7K
01:19Allosteric Proteins-ATCase
6.5K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
6.5K