帕图林生物降解由Rhodosporidiobolus ruineniae和Meyerozyma guilliermondii从水果中分离出来
Yidan Ji1, Sung-Yong Hong1, Jinhuan Qu1
1Department of Food and Nutrition, Hanyang University, Seoul, Republic of Korea.
MicrobiologyOpen
|January 6, 2026
概括
两个酵母菌株,Rhodosporidiobolus ruineniae和Meyerozyma guilliermondii,可以降解水果中的真菌毒素patulin (PAT). 它们将PAT转化为毒性较低的化合物 (E) - 基醇,为食品安全应用提供了潜力.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 菌类学 菌类学是指菌类学.
背景情况:
- 帕图林 (PAT) 是一种毒性真菌毒素,通常在果中发现.
- 控制PAT污染对食品安全和公共卫生至关重要.
研究的目的:
- 为了隔离和识别能够降解素的酵母菌株.
- 调查由选定的酵母菌株降解帕图林的最佳条件和机制.
- 评估这些酵母在控制水果中的帕图林方面的潜在应用.
主要方法:
- 从受污染的水果 (桃子和果) 中分离酵母菌株.
- 通过改变化时间,温度和初始度来优化帕图林降解.
- 使用细胞壁,细胞外和细胞内部分,球状质体和细胞内酶,分析帕图林降解机制.
- 使用LC/MS/MS识别帕图林生物降解产品.
主要成果:
- 确定了Rhodosporidiobolus ruineniae和Meyerozyma guilliermondii作为有效的病原蛋白降解剂.
- 这两种菌株在60小时内在35°C下降解了1μg/ml的病原蛋白,低于法规限制 (50μg/L).
- 帕图林降解发生在细胞内,而这种能力在M. guilliermondii中是可诱导的.
- 鉴定出 (E) - 亚斯基醇是唯一的生物降解产物.
结论:
- R. ruineniae和M. guilliermondii有效地降解了病原蛋白,产生了毒性较低的化合物 (E) -ascladiol.
- 通过这些酵母的细胞内质降解机制得到证实.
- 这些发现支持这些酵母菌株的实际应用,用于控制果和桃子等水果中的帕图林污染.
关键词:
(E) -阿斯克拉迪奥尔 (E) -阿斯克拉迪奥尔梅耶罗兹玛吉利尔蒙迪的世界rhodosporidiobolus 石灰岩 石灰岩 石灰岩 石灰岩 石灰岩 石灰岩 石灰岩生物降解 生物降解帕图林 (Patulin) 是一种蛋白质.酵母酵母是一种酵母.更多相关视频
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