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来自印度Bt分离的新型编码子优化cry1Ac34基因在转基因烟草中对Helicopoverpa armigera产生抵抗力

Anupma Singh¹, G G K Gouthami¹, V Rakesh^2,3

¹ICAR-National Institute for Plant Biotechnology, IARI Campus, New Delhi, 110012, India.

Archives of microbiology

|January 6, 2026

概括

来自Bacillus thuringiensis (Bt) 的新基因cry1Ac34显示出对转基因烟草植物中的Helicoverpa armigera的高疗效. 这为农作物抗虫管理提供了一个有希望的解决方案.

科学领域:

植物生物技术植物生物技术
分子昆虫学分子昆虫学
农作物保护农作物保护

背景情况:

在转基因作物中增加了Helicoverpa armigera对现有的Bacillus thuringiensis (Bt) 基因的耐药性.
需要新型杀虫基因来管理这一重要的农业害虫.
在农业中开发有效的害虫防治新策略.

研究的目的:

为了评估转基因尼科蒂亚纳烟草中cry1Ac34基因的植入疗效.
评估cry1Ac34作为一种针对Helicoverpa armigera的工具的潜力.
为了研究cry1Ac34在植物中的表达和杀虫活性.

主要方法:

尼科蒂亚纳烟草与cry1Ac34基因的农业细菌介导的转化.
分子分析包括PCR,南方涂抹和qRT-PCR用于基因确认.
使用Helicoverpa armigera幼虫进行昆虫生物测试,并对中肠组织进行基因病理检查.

主要成果:

成功生成和分子确认表达cry1Ac34.4的T1转基因烟草系.
在被转基因叶子养的H. armigera幼虫中,死亡率高 (高达95%).
观察到的次致命效应包括幼虫生长减少和中肠组织损伤.

结论:

编码子优化的cry1Ac34基因在转基因烟草中得到有效表达,并赋予了显著的昆虫耐药性.
cry1Ac34表现出强大的杀虫活性和对控制Helicoverpa armigera的潜力.
这种基因代表了开发新的耐昆虫转基因作物的有希望的候选人,以对抗害虫耐药性.

关键词:

细菌 thuringiensis 这种疾病.这是一架直升飞机的直升机pa armigera.cry1Ac34 基因基因基因基因编码优化 - 编码优化管理昆虫耐药性管理昆虫耐药性可持续的害虫管理可持续的害虫管理转基因开发的转基因开发

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