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Updated: Jan 13, 2026

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通过基于qRT-PCR的绝对量化,开发SCAR和快速检测黑色的YMV耐药基因型

Priyanka Halldakeri1, Amar A Sakure2, Sushil Kumar3

  • 1Department of Genetics and Plant Breeding, Anand Agricultural University, Anand, Gujarat, India.

Molecular biology reports
|January 6, 2026
PubMed
概括

研究人员确定了一种DNA标记物SCAR15,与黄色马赛克病毒 (YMV) 在黑色中抵抗有关. 这种标志物有助于培育抗YMV的黑色草品种,这对于提高作物产量至关重要.

关键词:
黑色的奶奶是什么意思 黑色的奶奶是什么意思在RAPD中,使用的是RAPD.这是RT-PCR.这是一个可怕的恐惧.在YMV中,YMV是YMV.

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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