纳米颗粒的细胞吸收是通过基于整合素的对细胞外基质的粘附来调节的
Sailesti Joshi1, Ananya Naha1, Justice Ene1
1Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University, 2525 Pottsdamer Street, Tallahassee, Florida 32310, United States.
ACS applied materials & interfaces
|January 6, 2026
概括
基板上的细胞外矩阵涂层显著影响细胞如何与治疗纳米粒子 (PLGA) 相互作用和吸收. 特定的涂层,如纤维蛋白和原,通过促进细胞粘附和细胞骨组织来增强纳米颗粒的吸收,这对再生医学至关重要.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 纳米技术纳米技术
- 再生医学是一种再生医学.
背景情况:
- 治疗性纳米粒子传递对于各种生物医学应用至关重要,包括药物传递和组织工程.
- 纳米粒子吸收受到细胞基质相互作用的影响,特别是细胞外基质 (ECM) 和细胞机械感知.
- 细胞-ECM相互作用调节整合素信号传递,焦点粘附形成和细胞骨动力学,影响纳米粒子内部化.
研究的目的:
- 研究不同ECM和ECM模拟涂层如何影响聚乳糖合糖酸 (PLGA) 纳米颗粒的吸收.
- 确定细胞基质相互作用,特别是焦点粘附形成和细胞骨组织在纳米粒子吸收中的作用.
- 为了比较不同细胞类型与不同焦点粘附成熟度的纳米粒子吸收.
主要方法:
- 使用散射式石英晶体微平衡 (QCM-D) 和圆测量,对ECM涂层 (原I,纤维素,拉米因,氨酸,聚氨酸) 的表征.
- 对涂层基板上的细胞增殖,扩散和焦点粘附形成的评估.
- 通过活细胞成像和利用细胞素-D进行吸收研究来量化PLGA纳米颗粒吸收,以探测actin细胞骨架依赖性.
主要成果:
- 纤维菌素 (FN) 和原I (COL) 涂层显著增强了细胞粘附,增殖和焦点粘附形成,从而导致更高的纳米颗粒吸收.
- 氨酸 (HA) 和氨酸 (LM) 涂层导致细胞粘附和纳米粒子吸收减少.
- 与巨细胞相比,具有更成熟的焦点粘附 (纤维细胞,ASC) 的细胞类型表现出更高的纳米粒子吸收率 (RAW264.7).
- 纳米颗粒的吸收取决于actin细胞骨架,这表明actin依赖性内细胞通路的参与.
结论:
- 电子介质 (ECM) 涂层特性和细胞类型特定的焦点粘附形成极大地调节了基质介导的纳米粒子吸收.
- FN和COL涂层促进了持续的纳米粒子吸收,而LM显示了快速的早期吸收,突出显示了动态的吸收动力学.
- 这些发现为设计用于再生医学和治疗应用的优化基质纳米粒子传递系统提供了洞察力.
相关概念视频
Intracellular Signaling Affects Focal Adhesions
3.5K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
3.5K
Overview of Cell-Matrix Interactions
8.8K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
8.8K
Integrins
5.2K
Animal and protozoan cells do not have cell walls to help maintain shape and provide structural stability. Instead, these eukaryotic cells secrete a sticky mass of carbohydrates and proteins into the spaces between adjacent cells. This network of proteins and molecules is called an extracellular matrix or ECM.
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...
5.2K
Activation of Integrins
4.9K
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding...
4.9K
Selectins
4.1K
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
4.1K
Cell-matrix's Response to Mechanical Forces
3.4K
In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue.
Anchoring junctions mechanically attach a cell to the...
Anchoring junctions mechanically attach a cell to the...
3.4K


