相关实验视频
Updated: May 12, 2026
06:11
A High-throughput Shigella-specific Bactericidal Assay
Published on: February 27, 2019
12.4K
控制食源性病原体,Shigella flexneri 1457通过使用植物聚纳灵宁的植物多
Vasudevan Ramya1, Singaravel Mahalakshmi1, Elumalai Rajalakshmi1
1Molecular Genetics Laboratory, Department of Genetic Engineering, School of Bioengineering, College of Engineering and Technology, SRM Institute of Science and Technology, Chengalpattu, Tamil Nadu, India.
概括
纳灵宁是一种植物衍生的黄胺,有效地抑制了Shigella flexneri的生长和生物膜的形成. 这种天然化合物显示出作为细菌痢疾的替代治疗方法的潜力,减少死细胞和降低关键毒性基因的调节.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
背景情况:
- 由Shigella flexneri引起的石病和细菌性痢疾是严重的健康问题.
- 目前的抗生素治疗因耐药性和健康问题而面临局限性,需要新的治疗策略.
- 多,特别是黄,表现出有希望的抗菌性质,并可以干扰细菌生物膜的形成.
研究的目的:
- 为了研究纳灵宁对Shigella flexneri的疗效.
- 评估纳灵宁对细菌生长,生物膜形成和毒性因子的影响.
主要方法:
- 最低抑制度的确定.
- 生物膜抑制测定.生物膜抑制测定.
- 扫描电子显微镜 (SEM) 用于形态分析.
- 光激活细胞分类 (FACS) 用于细胞活力评估.
- 定量逆转录PCR (qRT-PCR) 用于基因表达分析.
主要成果:
- 纳灵宁在1000μg/ml的剂量下抑制了Shigella flexneri的生长和生物膜的形成.
- 在S.flexneri中,SEM揭示了用naringenin治疗的形态变化.
- 在纳林根因治疗后,FACS显示死亡细胞增加了87.76%.
- qRT-PCR显示了粘附,定数感应和生物膜发育基因的下调.
结论:
- 纳灵宁显示出显著的抗菌和抗生物膜活性,可以对抗Shigella flexneri.
- 纳灵宁破坏细菌形态,增加细胞死亡,这表明它具有强大的抗菌机制.
- 关键毒性基因的降低调节突出显示了naringenin作为治疗shigellosis的治疗剂的潜力.
相关概念视频
01:29Methods for Controlling Microbial Growth
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
01:28Biological Methods for Microbial Control
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
01:28Gene Regulation in Microbial Communities: Quorum Sensing
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
01:18Bacterial Gastroenteritis
Bacterial gastroenteritis, characterized by diarrhea, abdominal cramps, and vomiting, is often caused by ingestion of contaminated food or water and is frequently associated with pathogenic Escherichia coli strains. These microbes exploit two principal mechanisms to inflict disease.Shiga toxin–producing E. coli, also referred to as STEC—notably O157:H7—release Shiga toxins that target ribosomes, blocking protein synthesis. The B subunit of the toxin binds the host glycolipid receptor...