基于PacBio测序和Hi-C技术的Nemipterus japonicus的染色体级基因组组装
Feng-Ling Lei1, Ren-Xie Wu2, Fan-Li Long1
1College of Fisheries, Guangdong Ocean University, Zhanjiang, 524088, China.
Scientific data
|January 6, 2026
概括
这项研究介绍了第一个高质量的染色体级基因组组合,用于经济重要鱼类,日本鱼. 这种基因组资源有助于理解尼米类鱼的遗传进化.
科学领域:
- 基因组学就是基因组学.
- 海洋生物学 海洋生物学
- 渔业 科学 渔业 科学
背景情况:
- 尼米 (Nemipterus japonicus) 是一种在印度西太平洋地区的商业价值的底生鱼.
- 目前N. japonicus的基因组数据有限,阻碍了进化和遗传研究.
研究的目的:
- 为了组装一个高质量的染色体水平的参考基因组为Nemipterus japonicus.
- 为N. japonicus和相关的Nemipterid物种提供基础基因组数据.
主要方法:
- 整合了Illumina,PacBio和Hi-C的测序技术.
- 染色体层次的基因组组装和注释.
- 对比基因组分析和积极选择分析.
主要成果:
- 一个723.27 Mb的基因组组件,一个30.96 Mb的N50支架,将97.04%的结合物固定在22个伪染色体上.
- 预测了23,514个编码蛋白质的基因和65,532个非编码RNA.
- 鉴定了218个扩展和1076个收缩的基因家族,以及163个积极选择的基因.
结论:
- 染色体水平的基因组为N. japonicus遗传研究提供了必不可少的资源.
- 这些发现为N. japonicus.的遗传进化和适应提供了洞察力.
- 这种基因组组件有助于未来对尼米鱼基因组学的研究.
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