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Updated: Jan 13, 2026

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活体拉曼光谱用于实时生物化学评估组织病理学和生理学

Ezekiel J Haugen^1,2, Rekha Gautam^3,4, Andrea K Locke^3,5,6

¹Vanderbilt Biophotonics Center, Vanderbilt University, Nashville, TN, USA. ezekiel.haugen@vanderbilt.edu.

Nature protocols

|January 6, 2026

概括

这项研究引入了一个标准化的协议,用于体内拉曼光谱 (RS),这是用于组织分析的无标签技术. 该协议旨在提高RS的可靠性和采用性,用于诊断和研究.

科学领域:

生物医学光学生物医学光学
频谱学是一种光谱学.
化学成像技术化学成像技术

背景情况:

在体内拉曼光谱 (RS) 提供无标签的实时组织生物化学分析.
它对诊断和外科指导的潜力很大,但由于缺乏标准化而受到限制.

研究的目的:

为体内RS测量建立一个全面的,逐步的协议.
为了促进更广泛的采用,并提高体内RS研究的可复制性.

主要方法:

仪器选择,对齐和校准的详细指南.
在体内数据收集,光谱处理和分析的标准化程序.
针对弱信号和自发光等常见挑战的故障排除策略.

主要成果:

一个标准化的协议,涵盖了体内RS应用的所有方面.
针对皮肤,子宫,食道和结肠的具体指导,可适应其他器官.
实践参考的示例参数和光谱输出.

结论:

开发的协议提高了体内RS研究的可重复性.
它作为扩大RS作为研究和临床工具的使用的关键指南.

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Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.