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Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
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相关实验视频

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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核结合复合体调节细胞下RNA处理和对编码和非编码RNA的监控.

Diep R Ganguly1, Brian D Gregory1

  • 1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, United States.

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概括
此摘要是机器生成的。

这项研究揭示了植物细胞核和细胞质中的各种RNA裂变事件,揭示了核结合复合体在RNA处理和监测中的新角色.

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科学领域:

  • 分子生物学分子生物学
  • 植物科学 植物科学
  • 基因组学就是基因组学.

背景情况:

  • RNA裂变对于RNA处理和调节至关重要.
  • 现有的分析RNA分裂的方法是有限的,主要关注细胞质事件,忽视亚细胞差异.

研究的目的:

  • 为了研究Arabidopsis thaliana.在不同细胞区 (细胞核和细胞质) 内的全基因组RNA裂变事件.
  • 为了识别在植物核中发生的新型RNA裂变和处理事件.

主要方法:

  • 连接细胞下RNA分离与降解组概况.
  • 分析RNA裂变模式在分离的核等离子体和细胞等离子体中.

主要成果:

  • 使者RNA (mRNA) 裂变在细胞质部分中占主导地位.
  • 鉴定出了广泛的核质丰富RNA裂变事件,包括mRNA前裂变和非编码RNA的处理 (microRNA,核质RNA,小核质RNA,增强剂关联RNA,逆转移素衍生的RNA).
  • CAP-BINDING PROTEIN80/ABA HYPERSENSITIVE1参与了围核细胞质中的mRNA监测和核质RNAs的稳定.

结论:

  • RNA分裂在植物细胞内高度细分,在细胞核和细胞质中发生不同的事件.
  • 核结合复合体在核等离子体RNA处理和细胞等离子体mRNA监测中发挥着多方面的作用.