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Updated: Jan 13, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
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在I-F3型CRISPR-Cas效应器的PAM远部位进行序列结构重组,使RNA引导DNA转换成为可能

Kazuki Ishihara1, Shunsuke Matsumoto1, Christoph Gerle2

  • 1Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan.

Nucleic acids research
|January 7, 2026
PubMed
概括

与CRISPR相关的转体质 (CAST) 使用RNA引导的DNA结合进行插入. 新的结构揭示了Cas8/5如何识别原空间体相邻动机 (PAM) 并曲DNA,指导转位.

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