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模拟癌症相关的纤维细胞可塑性和乳腺癌相互作用,使用患者衍生的支架

Santiago Ruiz-Martínez¹, Anna Gustafsson¹, Elin Johansson¹

¹Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, Sahlgrenska Center for Cancer Research, University of Gothenburg, Gothenburg 41390, Sweden.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

|January 7, 2026

概括

来自患者的支架揭示了癌症相关纤维细胞 (CAF) 与瘤微环境的相互作用. 这些模型表明,CAFs通过改变瘤细胞和细胞外基质来影响乳腺癌的进展,突出显示动态的瘤-肌瘤交叉声.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物材料是一种生物材料.

背景情况:

瘤微环境 (TME) 显著影响乳腺癌的进展.
癌症相关纤维细胞 (CAF) 是TME的关键参与者,影响瘤行为.
了解纤维细胞激活和瘤-肌瘤相互作用对于治疗开发至关重要.

研究的目的:

通过使用患者衍生支架 (PDSs) 调查纤维细胞激活和瘤-流体交叉交互.
模拟细胞外矩阵 (ECM) 架构对纤维细胞行为和乳腺癌进展的影响.
在TME中剖析CAF异质性和ECM驱动的调制.

主要方法:

使用了63个患者衍生的支架 (PDS),保留了原生ECM架构.
进行质谱分析以分析母体组合并识别CAF类丰富.
对乳腺癌细胞和纤维细胞进行过井和直接共同培养实验.

主要成果:

在与瘤等级,复发和CAF亚型 (myCAFs, iCAFs) 相关的PDS中确定了不同的母体组.
在PDS上表现出正常纤维细胞的渐进激活,具有上调的CAF标记物和ECM重塑基因.
展示了双向交叉:癌细胞影响了纤维细胞IL6分泌,而PDS培养的纤维细胞以亚型依赖的方式调节了癌细胞的增殖和干细胞.

结论:

PDS提供了一个生理学上相关的平台,用于研究瘤-肌瘤相互作用和CAF异质性.
纤维细胞激活和ECM重塑是受空间背景和癌细胞信号影响的动态过程.
结果强调了TME在乳腺癌进展中的重要性,并建议PDS用于未来的研究.

关键词:

乳腺癌是什么? 乳腺癌是什么与癌症相关的纤维细胞.患者衍生的支架是患者衍生的支架瘤微环境是一个微环境.

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