相关实验视频

Updated: May 4, 2026

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Plant-Microbe Interaction: Transcriptional Response of Bacillus Mycoides to Potato Root Exudates

Published on: July 2, 2018

从Bacillus subtilis SBM_1的固态发酵大豆粉中产生的转录组分析

Dandan Liu^1,2, Junsong Zhu³, Yiting Guo³

¹Shandong Food Ferment Industry Research & Design Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.

Journal of the science of food and agriculture

|January 7, 2026

概括

细菌细菌SBM_1通过对蛋白酶和合成集群进行上调来增强固态发酵 (SSF) 期间的产量. 质通过ABC载体分泌和运输,这对于无抗生素料开发至关重要.

科学领域:

微生物学和生物技术
生物化学生物化学
分子生物学分子生物学

背景情况:

用质丰富的微生物发酵料为无抗生素料开发提供了潜力.
在固态发酵 (SSF) 过程中了解生成机制是过程优化的关键.
这项研究调查了在SSF期间Bacillus subtilis SBM_1的转录变化,以阐明的生产机制.

研究的目的:

在SSF期间调查 Bacillus subtilis SBM_1 中的转录变化.
探索B. subtilis SBM_1.1.中的化物生产的机制.
确定关键的基因和涉及合成和分泌的途径.

主要方法:

细菌细菌的固态发酵 (SSF) SBM_1.1.
使用差异基因表达的转录分析.
丰富途径和基因集群的生物信息分析.

主要成果:

差异表达基因 (DEGs) 在新陈代谢,ABC载体和氨基酸生物合成途径中得到丰富.
细胞外蛋白酶合成和分泌的基因被上调,促进大豆蛋白质的水解.
B. subtilis SBM_1 合成和分泌了核糖体合成和转化后修饰 (RiPP) 和非核糖体 (NRP),包括氨酸和亚氨酸.

结论:

B. subtilis SBM_1在SSF过程中合成和分泌细胞外蛋白酶,从大豆蛋白中产生.
合成的体通过ABC输送通路出口,导致体积累.
这些发现支持在无抗生素的动物营养中使用发酵料.

关键词:

细菌细菌SBM_1 SBM_1 细菌细菌这是一种类.固态发酵的固态发酵方式翻译学翻译学翻译学翻译学

更多相关视频

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi

Published on: June 6, 2025

相关概念视频

Production of Antibiotics

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

Production of Pharmaceuticals

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...

Production of Biopesticides

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...