研究tef (Eragrostis tef) 在人体THP-1单细胞中的抗氧化性能的分子机制

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Anastasia V Romanov¹, Tyler N Graf², Warren Vidar²

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概括

棕色Tef谷物提取物通过激活Nrf2信号通路来增强细胞抗氧化剂. 这项研究确定了关键的植物化学物质,如Vernolic和α-linolenic酸,负责这种抗氧化剂增强.

科学领域:

营养生物化学营养生物化学
分子生物学分子生物学
植物化学植物化学

背景情况:

棕色 (Eragrostis tef) 谷物提取物在单细胞中提升细胞谷氨 (GSH).
在此之前,人们还不知道Tef的抗氧化作用背后的精确分子机制.

研究的目的:

为了阐明Tef谷物提取物增强抗氧化剂水平的分子途径.
为了确定Tef的特定植物化学物质,负责这些影响.

主要方法:

使用了具有 Nrf2-抗氧化剂反应元素-露西法酶记者系统的 HEK293 细胞来评估通路激活.
进行RNA测序 (RNA-seq) 来分析tef提取物诱导的基因表达变化.
采用液体染色学-质谱学 (LC-MS) 来识别和量化活性 tef 分数中的化合物.

主要成果:

Tef 提取物诱导 luciferase 活性增加了 19 倍,证实了 Nrf2-抗氧化剂通路的激活.
RNA-seq确定了87个差异表达的基因,包括由Nrf2调节的基因,如费里重链假基因和瘤亡因子 (TNF) -α.
LC-MS分析显示,活性 tef 分数中和α-烯酸的丰富程度显著 (超过60倍).

结论:

棕色的Tef粒含有植物化学物质,可以激活Nrf2信号通路.
维诺酸和α-烯酸是促进tef抗氧化剂增强功能的关键化合物.
通过Nrf2通路促进细胞抗氧化防御,TEF的消费可能会带来好处.

关键词:

具有抗氧化活性的抗氧化剂.时代的花是没有的.葡萄糖氨酸 (Glutathione) 是一种路西法拉斯测试试验 Nrf2 没有任何问题.在THP-1单细胞中.对转录组进行分析.

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