细胞启动因子3d通过综合应激反应调节环境依赖的疼痛过敏性
Subhaan M Mian1, Sera I Nakisli1, Brodie J Woodall1
1Center for Advanced Pain Studies and Department of Neuroscience, School of Behavioral and Brain Sciences, University of Texas at Dallas, Richardson, TX 75080.
bioRxiv : the preprint server for biology
|January 7, 2026
概括
细胞转化启动因子3亚单元D (eIF3d) 缺乏会降低压力诱导的疼痛模型中的过敏性. 然而,它可能会增强中心敏感性,这表明在疼痛处理中具有上下文依赖的作用.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
背景情况:
- 细胞转化启动因子3亚单元D (eIF3d) 是一种非正规的结蛋白,在压力期间参与选择性mRNA转化.
- 它在疼痛处理中的作用在很大程度上仍未被探索.
研究的目的:
- 通过使用异合体eIF3d淘汰赛小鼠模型,研究eIF3d对疼痛处理的贡献.
- 为了确定eIF3d是否在各种压力条件下调节可感知可塑性.
主要方法:
- 通过测量eIF3d mRNA和蛋白质水平来验证eIF3d异构体淘汰 (eIF3d+/-) 鼠标模型.
- 在eIF3d+/-和野生型 (WT) 小鼠中评估基线疼痛行为 (机械,热,冷,自发).
- 在外周炎症 (甲基素,IL-6,脚切口) 和中央敏感化 (形式林试验,EAE) 模型中评估疼痛反应.
主要成果:
- eIF3d+/-小鼠在炎症和代谢应激模型中显著减少了机械和寒冷过敏.
- 在eIF3d+/-小鼠中观察到甲试验中增加了第二阶段的有害行为,这表明加强了中央敏感性.
- 在eIF3d+/-和WT小鼠之间,高过敏性原始化和EAE诱导是可比的.
结论:
- 根据特定的压力条件,eIF3d可以选择性调节感知可塑性.
- 这些发现表明,eIF3d在调节炎症性疼痛和中央敏感性方面具有上下文相关的作用.
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