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定义RNase E在真菌细菌降解体样网络中的作用

Abigail R Rapiejko¹, Ying Zhou¹, Vidhyadhar Nandana²

¹Department of Biology & Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts, 01609, USA.

bioRxiv : the preprint server for biology

|January 7, 2026

概括

结核菌的mRNA降解涉及核糖核酶 (RNases). 结核菌的mRNA降解包括核糖核酶 (RNases). RNase E的内在失序区域 (IDR) 是RNA结合和酶相互作用的关键,可以在压力时适应.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

mRNA降解对于*Mycobacterium tuberculosis*中调节基因表达至关重要.
几种核糖核酶 (RNases) 协调mRNA衰变,但它们的相互作用尚不清楚.
速度限制酶RNase E具有潜在参与这些相互作用的内在失序区域 (IDR).

研究的目的:

为了阐明主要的真菌细菌mRNA降解酶之间的相互作用.
为了定义RNase E在*Mycolicibacterium smegmatis*中的两个IDR的功能性作用.
了解这些相互作用如何促进细胞适应性.

主要方法:

研究的mRNA降解率和转录丰度 *in vivo*.
评估了催化活性,RNA结合和酶相互作用 *in vitro*.
检查了mRNA降解酶的细胞下局部 *in vivo*.

主要成果:

RNase E的IDRs对*in vivo*mRNA降解率有差异性影响,但在稳定状态转录水平上显示功能冗余.
IDRs不是催化剂,但对于RNA结合和与PNPase,RNase J和RhlE1 *in vitro* 的相互作用至关重要.
在体内*,IDRs在酶同定位中起到较小的作用,表明冗余的定位机制.

结论:

提出了一个类似于真菌细菌降解体的网络模型.
这个网络是通过RNA降解酶和RNA之间的动态,短暂的相互作用来稳定.
压力会破坏这些相互作用,促进菌根菌体的生理适应性.

关键词:

在 RNase E 中.一些降解和降解.退化了类似体的网络.在mRNA衰变过程中.mRNA降解降解的过程在mRNA处理过程中.

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