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相关概念视频

Reporter Genes02:11

Reporter Genes

12.9K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.7K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.6K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.6K
What is Gene Expression?01:42

What is Gene Expression?

194.3K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
194.3K
What is Gene Expression?01:36

What is Gene Expression?

10.9K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
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相关实验视频

Updated: Jan 13, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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可通过适应剂调节的体内基因表达.

Jack Bryant1, Laura Herron1, Yesh Doctor1

  • 1Department of Bioengineering, University of California San Diego, CA, USA.

bioRxiv : the preprint server for biology
|January 7, 2026
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种小型的RNA开关,称为适应体 (ADAR可调节的aptamer),用于精确控制基因表达. 该系统通过利用小分子和内源RNA编辑来实现可调节的基因疗法,显示出逆转小鼠肥胖的前景.

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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科学领域:

  • 生物技术是生物技术.
  • 分子生物学分子生物学
  • 基因治疗 基因治疗

背景情况:

  • 目前用于基因治疗的诱导基因表达系统面临诸如大尺寸,短诱导时间和免疫调节器等挑战,阻碍了体内应用.
  • 实现转基因表达的精确,可逆控制对于基因治疗的安全性和有效性至关重要.

研究的目的:

  • 开发一种新的,紧的RNA开关,以精确和可逆地控制转基因表达.
  • 创建一个临床兼容的系统,用于使用内源性RNA编辑调节基因疗法.

主要方法:

  • 设计了一种名为adaptamer的紧型RNA开关 (<120 bp),将一个小分子响应的aptamer与ADAR介导的RNA编辑集成在一起.
  • 在包括人类T细胞在内的各种细胞系中证明了适应剂的功能.
  • 在小鼠中利用腺相关病毒 (AAV) 传递来通过适应体系统控制FGF21表达.

主要成果:

  • 适配体系统能够通过后转录机制,通过精确的,小分子依赖的蛋白质翻译调制.
  • 适应体在多个细胞系中显示出高功能性,包括人类T细胞.
  • 在体内,AAV传递的适应体控制的FGF21表达导致显著的代谢重塑,增加能量消耗,并在小鼠中逆转肥胖.

结论:

  • 适应器代表了一个最小的,可编程的RNA开关,用于可调节的遗传药物.
  • 该系统提供了一种临床兼容的方法,用于在基因治疗中安全部署类蛋白和剂量限制蛋白质.