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Overview Of Cell Separation And Isolation01:20

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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基于形状的微流体分离细胞和粒子:最近的进展和未来的前景.

Muhammad Soban Khan1, Raihan Hadi Julio1, Mushtaq Ali1

  • 1Department of Mechanical Engineering, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Republic of Korea. jinsoopark@jnu.ac.kr.

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此摘要是机器生成的。

基于形状的微流体分离提供了精确的颗粒隔离,优于生物医学和材料应用的基于尺寸的方法. 本综述详细介绍了选择形状的细胞和粒子分类的被动和活性技术的进展.

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科学领域:

  • 微流体学 微流体学
  • 生物技术是生物技术.
  • 材料科学 材料科学 材料科学

背景情况:

  • 基于尺寸的传统颗粒分离方法是有限的.
  • 基于形状的分离对于隔离具有相同体积但不同的形态的粒子至关重要,对于生物医学应用 (如病理细胞隔离) 至关重要.
  • 微流体平台为基于形状的分离提供了一个强大的,但尚未开发的战略.

研究的目的:

  • 提供对微流体平台的最新进展进行形状选择性分离的全面概述.
  • 批判性地分析被动和主动的微流体技术,以进行形状歧视.
  • 突出基于形状的微流体分离的进展,挑战和未来方向.

主要方法:

  • 对被动微流体系统的审查:确定性的横向位移,压缩流量分成,惯性和粘弹性微流体.
  • 对活性微流体系统的审查:电电泳,磁泳,光泳和声泳.
  • 分析底层机制,技术进步和形状敏感性的实验/计算方法.

主要成果:

  • 最近的进展显示,高纯度超过95%,以形状为基础的分类效率超过90%.
  • 吞吐量从每分钟微升到毫升不等,取决于设备配置.
  • 技术利用水力动力学相互作用或外部场调节粒子轨迹基于几何异型.

结论:

  • 基于形状的微流体分离是诊断,治疗和材料科学中高精度颗粒隔离的一个有希望的策略.
  • 挑战包括模拟复杂的粒子行为 (旋转,对齐,可变形) 和需要集成控制和优化.
  • 未来的方向包括推进可扩展,高精度的基于形状的分离技术.