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草 S100A10a/10b,两种皮肤伤口诱导的基因,增强上皮细胞的增殖和迁移 in vitro

Jinzhi Yu¹, Naixuan Wang¹, Lijun Liu¹

¹School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, People's Republic of China.

Fish physiology and biochemistry

|January 7, 2026

概括

草S100A10a和S100A10b蛋白质促进上皮细胞的增殖和迁移. 这些发现表明它们在鱼皮伤口愈合和修复过程中起着至关重要的作用.

科学领域:

鱼类生物学鱼类生物学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

S100A10蛋白家族成员在哺乳动物中得到了很好的研究.
两种相似的S100A10基因 (S100A10a和S100A10b) 在远程生物中进化.
鱼类S100A10基因的功能作用在很大程度上是未知的.

研究的目的:

识别和描述草S100A10的同类物种 (gcS100A10a和gcS100A10b).
研究gcS100A10a和gcS100A10b在鱼上皮细胞中的功能.
确定这些蛋白质在鱼皮伤口愈合中的作用.

主要方法:

实时定量PCR用于基因表达分析.
在Epithelioma papulosum cyprini (EPC) 细胞中进行过度表达研究.
细胞活力,增殖 (PCNA),DNA合成和细胞周期测试.
用矩阵金属蛋白酶 (MMP) 抑制剂进行细胞迁移测定.
细胞外和细胞内MMP活性评估.

主要成果:

在草皮肤中高度表达的gcS100A10a和gcS100A10bmRNA在创伤后升级.
过度表达gcS100A10a/gcS100A10b可以提高EPC细胞的活力,增殖和DNA合成.
这两种蛋白质都加速了EPC细胞周期向S和G2/M阶段的进展.
gcS100A10a/gcS100A10b促进EPC细胞迁移,涉及MMP活动.
过度表达增加了细胞外MMP活性,并减少了细胞内MMP积累.

结论:

gcS100A10a和gcS100A10b积极调节鱼类上皮细胞的增殖和迁移.
这些蛋白质在草皮肤中高度表达,并因受伤而引起.
结果表明gcS100A10a和gcS100A10b在鱼皮伤口愈合中的潜在作用.

关键词:

皮质细胞是上皮细胞.草鱼是一种草鱼.移民移民移民核武器的扩散在S100A1010中,使用的是S100A10.

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