通过LC-MS/MS对人类血清中的雌激素,孕,雄激素和皮质类固醇进行定量分析的敏感方法
Ersilia Bifulco1,2, Kai Triebner2,3, Francisco Gómez Real3
1Department of Biological Science, University of Bergen, Bergen, Norway.
The journal of applied laboratory medicine
|January 7, 2026
概括
一种新的液体染色学-双重质谱法 (LC-MS/MS) 方法准确地测量了人类血清中的11种类固醇激素. 这种敏感的测试非常适合大规模的生物库研究和荷尔蒙分析.
科学领域:
- 临床化学 临床化学
- 分析化学 分析化学
- 内分泌学 在内分泌学.
背景情况:
- 质谱 (MS) 与免疫测试相比,在类固醇激素的确定方面提供了更高的选择性和灵敏度.
- 现代MS仪器可以在小样本体积中测量皮科莫拉度,这对于生物库研究至关重要.
研究的目的:
- 开发和验证一种选择性和敏感的液体染色学-并联质谱法 (LC-MS/MS) 方法,用于量化人类血清中的11种类固醇激素.
- 评估开发的方法是否适用于大规模生物库研究.
主要方法:
- 机器人蛋白质沉和液体液体提取程序用于样品制备.
- 类固醇被分离在C18逆相柱上,在柱后注入氧化以增强电离.
- 用负离子和正离子模式进行多重反应监测进行了测量.
主要成果:
- 该方法实现了17β-雌激醇和雌激素等关键类固醇的低量化极限.
- 对于中等类固醇度,表现出极好的精度 (CVs 2.3%10.5%) 和准确性 (97%109%).
- 该试验显示出良好的线性 (r2 ≥0.9779) 和恢复 (83%114%),成功分析了921名女性的认证对照血清和类固醇配置文件.
结论:
- 成功开发了一种针对人血清中的11种类固醇激素的选择性,敏感和强大的LC-MS/MS方法.
- 经过验证的方法非常适合用于需要精确的荷尔蒙测量的生物库研究.
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