在人类肥胖症中,脏细胞衰老通过尿液细胞外囊泡检测到
Lei Zhang1, Mina Al-Saeedi2, Aleksandar Denic3
1Division of Nephrology and Hypertension, Mayo Clinic, Rochester, MN, USA; Department of Urology, Zhongda Hospital, Southeast University, Nanjing, China.
肥胖会触发细胞衰老,这是损伤的关键因素,即使功能正常. 这种细胞变化可以通过尿液细胞外囊 (UEV) 进行非侵入性检测.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學.
- 细胞生物学 细胞生物学
- 代谢性疾病 代谢性疾病
背景情况:
- 肥胖是造成功能障碍的一个重要危险因素.
- 细胞衰老和与衰老相关的分泌表型 (SASP) 导致早期损伤.
- 尿液细胞外囊泡 (uEVs) 提供了一种非侵入性方法来评估细胞应激.
研究的目的:
- 为了调查肥胖是否会诱导 podocyte 衰老.
- 为了确定 podocyte-derived uEVs 是否可以检测这种衰老.
- 为了将尿路标记与代谢和损伤指标相关联.
主要方法:
- 招募肥胖者 (OB) 和健康志愿者 (HV) 组.
- 通过流细胞测量分析了细胞特异标记 (PODXL+) 和衰老/SASP标记 (p16,MCP-1) 的UEV.
- 与代谢参数和尿损伤标志物 (KIM-1,NGAL,TNF-α,蛋白尿) 相关联的UEV发现.
- 在脏活检和量化尿路 podocytes 中验证了 podocyte 衰老.
主要成果:
- 与健康对照人群相比,肥胖个体显示出明显增加的衰老的 podocyte 衍生的 uEVs (P16+MCP-1+PODXL+) 分数.
- 这些升高的UEV与代谢功能障碍 (BMI,胰岛素抵抗) 和损伤标志物相关.
- 脏活检证实肥胖者细胞衰老的增加,尿细胞数量在肥胖群体中更高.
结论:
- 人类的肥胖会诱导细胞衰老,即使在功能保存的个体中也是如此.
- 衰老的 podocyte-derived uEVs 作为一个非侵入性的生物标志物,用于检测肥胖引起的脏压力.
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