QTL精细映射和功能分析显示,RS325845933在RPS6KB1的3'UTR中是影响Erhualian猪子大小的假定功能突变
Nengjing Jiang1, Mengru Ni1, Tianwei Zhou1
1Key Laboratory of Pig Genetic Resources Evaluation and Utilization (Nanjing), Ministry of Agriculture and Rural Affairs, Institute of Swine Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
International journal of biological macromolecules
|January 7, 2026
概括
研究人员在RPS6KB1基因中发现了一种基因标记物 (rs325845933),该基因对猪的子大小有显著影响. 这一发现为提高猪养殖计划的繁殖率提供了有价值的工具.
科学领域:
- 动物遗传学动物遗传学
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
背景情况:
- 垃圾大小是养猪的关键经济特征.
- 埃尔瓦尔猪表现出高繁殖率,与猪染色体12 (SSC12) 上的定量性质位置 (QTL) 有关.
研究的目的:
- 在SSC12上对子大小的QTL进行细化映射.
- 为了确定候选基因和功能变异影响猪的繁殖能力.
主要方法:
- 在SSC12上精确地绘制了子大小QTL的精细映射.
- 免疫组织化学检测卵巢中的p70S6K蛋白表达.
- 对p70S6K信号通路的功能分析.
- 在RPS6KB1.1.中识别和表征单核酸多态 (SNP).
- 对SNP与子大小的关联分析.
主要成果:
- 确定了RPS6KB1作为候选基因,p70S6K蛋白在卵巢theca细胞中表达.
- p70S6K调节雄激素合成,并通过PI3K/Akt/mTOR通路促进毛囊血管生成.
- 在RPS6KB1 3'UTR中的一个新型SNP (rs325845933) 与ssc-miR-7135-5p相互作用,调节基因表达.
- rs325845933与Erhualian,美国大白猪和加拿大大白猪的 litter 规模的增加显著相关.
结论:
- 在RPS6KB1中SNP rs325845933是一种影响猪繁殖的功能变异.
- 这种SNP可以被用作基因选择的分子标记物,以改善养猪计划中的子大小.
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