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Updated: Jan 13, 2026

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在蓝藻细菌中建立异质贝塔桑色素生物合成

Sayali S Hanamghar¹, David A Russo², Silas Busck Mellor³

¹Friedrich Schiller University Jena, Matthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Synthetic Biology of Photosynthetic Organisms, D-07743, Jena, Germany.

Metabolic engineering

|January 7, 2026

概括

在这项研究中,科学家利用蓝藻细菌生产贝塔桑,一种天然的黄色颜料. 优化条件显著提高了产量,为这些有价值的食品色素建立了可持续的微生物路线.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
微生物颜料的生产方法

背景情况:

贝塔,包括贝塔丁,是天然的颜料,被寻求作为合成食品染料的替代品.
目前从植物中提取的颜料由于颜料度低,因此效率低下.
可持续和可扩展的色素生产需求需要新的方法.

研究的目的:

开发一种微生物系统,用于在蓝藻细菌中产生光自营性贝塔桑丁.
为了设计一个异质的路径,用于betaxanthin生物合成.
优化代谢流量和培养条件,以提高色素产量.

主要方法:

在Synechocystis sp.中引入了两种酶通路 (CYP76AD1变体和DODA1). 在PCC 6803中.
使用反抗性AroGfbr. 的shikimate通路的代谢工程.
优化生长媒介和二氧化碳丰富用于种植.

主要成果:

最初的途径引入没有产生可检测的betaxanthins.
代谢调整导致了芳香氨基酸的积累和低氨酸-贝塔桑的生产.
优化栽培增加了标位165倍,达到18.2±5.1毫克L-1氨酸-betaxanthin.

结论:

在蓝藻细菌中建立了一种功能性微生物系统,用于生产betaxanthin.
在没有外源氨基酸补充的情况下实现了光自营养生产.
这项工作为自然色素生成提供了可扩展和可持续的方法.

关键词:

在 Betalain Betalain 的位置.贝塔克桑丁是一种贝塔克桑丁.蓝藻细菌是一种蓝藻细菌.细胞染色体P450的使用.石基马特通道的路径这就是Synechocystis.

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