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Updated: Jan 13, 2026

High-throughput Detection Method for Influenza Virus

High-throughput Detection Method for Influenza Virus

Published on: February 4, 2012

开发和应用实时RT-PCR试验,用于特定检测D型流感病毒

Jinfeng Wang¹, Ruiwen Li², Libing Liu¹

¹Technology Center of Shijiazhuang Customs, Shijiazhuang 050051, China.

Journal of virological methods

|January 7, 2026

概括

D型流感病毒 (IDV) 引起牛呼吸道疾病复合体 (BRDC). 一种新的实时RT-PCR检测准确地检测牛群中的IDV,确认其在中国河北省的存在.

科学领域:

兽医病毒学兽医病毒学
分子诊断学分子诊断
动物健康动物健康

背景情况:

在全球范围内,D型流感病毒 (IDV) 在各种动物物种中越来越多地被检测到.
IDV被认为是导致牛呼吸道疾病复合体 (BRDC) 的重要病原体.

研究的目的:

开发和验证用于IDV检测的高度特定和敏感的实时RT-PCR (RT-qPCR) 试验.
调查中国河北省牛群中IDV的流行情况.

主要方法:

设计了特定的原料和TaqMan探针,针对IDV的保存PB1基因.
验证了RT-qPCR试验的特异性,并确定了其检测极限 (100拷贝/反应).
在中国河北省 (2021-2022) 的417头牛鼻样本上进行了测试.

主要成果:

RT-qPCR测定显示IDV的高特异性,没有与其他BRDC病原体的交叉活性.
在被测试的牛鼻的7.91% (33/417) 中检测到IDV.
从阳性样本中对HEF基因的测序证实了具有高核酸同质性的D/Yama2019血统.

结论:

成功开发了一种强大的RT-qPCR试验,用于特定和敏感的IDV检测.
这项研究提供了第一个证据,证明中国河北省牛群中IDV的传播.
这些发现强调了IDV监测在牛群中的重要性.

关键词:

D/Yama2019 的血统是D/Yama2019 的血统这就是HEF基因.流感D型病毒感染者在PB1基因中,PB1基因被发现.实时RT-PCR实时RT-PCR的时间

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相关概念视频

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...