机体模拟和单细胞剖析揭示了莫亚莫亚疾病中的光滑肌肉细胞迁移
Shihao He1,2,3, Junze Zhang4,5, Xilong Wang4,5
1Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. heshihaoo@outlook.com.
Communications biology
|January 7, 2026
概括
莫亚莫亚病 (MMD) 涉及异常的血管生长. 研究人员发现,高蛋白 TUBA4A 和 TUBB4B 通过影响血管光滑肌细胞,为MMD进展做出贡献,从而提供潜在的治疗点.
科学领域:
- 神经科学是一个神经科学.
- 血管生物学 血管生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 莫亚莫亚病 (MMD) 是一种脑血管疾病,导致脑内动脉狭窄,导致中风.
- 目前尚不完全了解MMD的潜在病原体,这阻碍了有效的诊断和治疗.
研究的目的:
- 调查分子机制并确定莫亚莫亚病的潜在生物标志物和治疗点.
主要方法:
- 数据独立获取 (DIA) 血清上的蛋白质组学从MMD患者和对照组.
- 通过ELISA验证,从诱导多能干细胞 (iPSCs) 产生血管类器官,组织学染色,转录组学和单细胞RNA测序 (scRNA-seq).
主要成果:
- 在MMD患者的动脉中血管光滑肌细胞 (VSMC) 的异常积累.
- 在MMD血清中显著上调TUBA4A和TUBB4B.
- TUBA4A和TUBB4B通过GJA1/PI3K/AKT/KLF4通路促进VSMC的增殖,迁移和表型转换.
- 在MMD血管器官中,合成VSMCs增加,TUBA4A和TUBB4B的含量增加.
结论:
- 过度表达TUBA4A和TUBB4B有助于MMD的病态血管重塑.
- GJA1/PI3K/AKT/KLF4通路与MMD病变发生有关.
- TUBA4A和TUBB4B代表了MMD干预的潜在治疗点.
更多相关视频
09:06Quantitative Analysis of Cellular Composition in Advanced Atherosclerotic Lesions of Smooth Muscle Cell Lineage-Tracing Mice
Published on: February 20, 2019
8.8K
11:47Construction of a Human Aorta Smooth Muscle Cell Organ-On-A-Chip Model for Recapitulating Biomechanical Strain in the Aortic Wall
Published on: July 6, 2022
3.7K
相关概念视频
01:19Cell Migration
6.4K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.4K
01:09Cell Migration
18.6K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.6K
01:18Role of Myosin in Cell Migration
3.2K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
Myosin II is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.2K
01:32Cytoskeletal Coordination in Cell Migration
5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K
01:25Smooth Muscle Contraction
7.2K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
7.2K
01:35Cancer Cell Migration through Invadopodia
3.2K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.2K