皮动物中cis调节元素和染色质组织的深度保存揭示了动物基因组的祖先调节特征
Marta S Magri1,2,3, Danila Voronov4, Saoirse Foley5,6
1CABD (Centro Andaluz de Biología del Desarrollo), CSIC-Universidad Pablo de Olavide-Junta de Andalucía, Seville, Spain.
Nature ecology & evolution
|January 7, 2026
概括
皮动物在数百万年的时间里表现出保存的调节元素,类似于脊椎动物. 他们的3D基因组组织揭示了TAD类域,为动物调节进化提供了洞察力.
科学领域:
- 基因组学就是基因组学.
- 进化生物学 进化生物学
- 发展生物学 发展生物学
背景情况:
- 在非模型动物系中对调控基因组组织的有限知识.
- 和脊椎动物之间3D染色质组织和cis-regulatory element (CRE) 演变的对比趋势.
- 皮动物为研究基因组调节提供了独特的遗传学位置和较慢的分子进化.
研究的目的:
- 研究皮体调节基因组的进化和组织.
- 描述皮动物的3D染色质结构和CRE动态.
- 将皮的调节进化与其他动物血统进行比较.
主要方法:
- 使用PacBio和HiC数据,为Strongylocentrotus purpuratus (海) 和Patiria miniata (海星) 创建新的参考基因组组.
- 3D染色体架构的表征,包括TAD类域.
- 在开发过程中使用ATAC-seq.的CREs的分析.
- 跨进化时间尺度对CRE进化的比较分析.
主要成果:
- 皮动物拥有类似TAD的域,与CTCF图案定向无关,类似于.
- 在整个开发过程中识别和分析海和海星中数千种保存的CREs.
- 在皮动物中展示了类似于脊椎动物的CRE进化模式,其中的元素保存了数亿年.
结论:
- 体3D基因组组织与有共同的特征,而CRE进化则反映了脊椎动物.
- 跨越巨大的进化距离保存的CREs表明动物调节进化模式的古代起源.
- 这项研究为动物基因调节的深刻进化历史提供了关键的见解.
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