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相关概念视频

Ligand Binding Sites02:40

Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Signal Transduction: Overview01:26

Signal Transduction: Overview

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Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk.
Typically, signal transduction involves three...
11.4K
The Two-State Receptor Model01:29

The Two-State Receptor Model

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The two-state receptor model explains a drug's interaction with receptors, such as G protein-coupled receptors and ligand-gated ion channels, to induce or inhibit a biological response. When no natural ligands are present, a receptor exists in an equilibrium of inactive (Ri) and active (Ra) conformations. The inactive form does not produce a response, while the active form generates a basal effect known as constitutive activity.
The binding affinity of a drug determines its interaction with...
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Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

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Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
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Physiological Pharmacokinetic Models: Blood Flow-Limited Versus Diffusion-Limited Models00:57

Physiological Pharmacokinetic Models: Blood Flow-Limited Versus Diffusion-Limited Models

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Physiological pharmacokinetic models, often called flow-limited or perfusion models, typically assume a swift drug distribution between tissue and venous blood, creating a rapid drug equilibrium. This premise is based on the idea that drug diffusion is extremely fast, and the cell membrane presents no barrier to drug permeation. In this scenario, where no drug binding occurs, the drug concentration in the tissue equals that of the venous blood leaving the tissue. This greatly simplifies the...
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相关实验视频

Updated: Jan 13, 2026

Author Spotlight: Exploring Cellular Processes by Modeling Ligands in Cryo-EM Maps
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通过连接体扩散和传输模型推进空间细胞通信推断.

Jiating Yu1, Jinyue Zhao2, Tao Ren3,4

  • 1School of Mathematics and Statistics, Nanjing University of Information Science & Technology, Nanjing, China.

Communications biology
|January 7, 2026
PubMed
概括

SCILD通过使用一种新的连接物扩散模型,以单细胞分辨率推断空间细胞通信. 这个工具准确地捕捉复杂的信号动态,并预测目标基因,推进空间转录学研究.

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Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT
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科学领域:

  • 分子生物学分子生物学
  • 系统生物学 系统生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 细胞-细胞通信对于多细胞生物来说至关重要,依赖于连接体-受体相互作用.
  • 传统的研究细胞通信的方法缺乏空间分辨率,容易出现错误.
  • 空间转录学能够通过精确的空间信息来分析通信事件.

研究的目的:

  • 开发一种新的计算框架,以单细胞分辨率推断空间细胞通信.
  • 整合联体扩散,竞争性结合和运输动态到一个统一的模型中.
  • 为了预测由联体受体相互作用调节的下游目标基因.

主要方法:

  • 推出了基于优化的框架SCILD (空间细胞通信推理与联结体扩散和传输模型).
  • 模拟蜂通信作为一个带有潜在损失的货物运输系统,包括连接体扩散和竞争性结合.
  • 利用神经网络建模和in silico扰动用于目标基因预测.

主要成果:

  • 在单个细胞层面上,SCILD准确地捕捉了竞争性的沟通动态.
  • 确定了生物相关的联体受体标志物,驱动域特异信号.
  • 解决了子域特定的通信模式和强有力的预测目标基因.
  • 与外部数据库对比的验证结果.

结论:

  • SCILD是一个多功能和强大的工具,用于空间细胞通信研究.
  • 该框架增强了对复杂生物系统中细胞-细胞信号传递的理解.
  • SCILD提供了以前无法实现的通信动态的高分辨率洞察力.