评估皮肤衰老的基于转录组的工具揭示了干细胞衍生的细胞外囊泡对UVB诱导的衰老的改善作用
Jinpeng Xie1,2, Lihong Wang1,2, Shuaifei Zhao1,2
1Oujiang Laboratory (Zhejiang Lab for Regenerative Medicine, Vision and Brain Health), School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, P. R. China.
BMC genomics
|January 7, 2026
概括
我们开发了SkinAGE,一个深度神经网络的衰老钟,用基因表达来测量生物衰老. SkinAGE准确量化细胞衰老,并评估抗衰老治疗的有效性,例如人类胚胎干细胞衍生的细胞外囊 (hESC-EVs).
科学领域:
- 生物遗传学 生物遗传学
- 计算生物学 计算生物学
- 皮肤病学 皮肤病学
背景情况:
- 量化生物衰老对于理解与年龄相关的功能衰退至关重要.
- 评估抗衰老干预措施的有效性需要可靠的衰老生物标志物.
- 基于转录基因组的方法提供了对细胞衰老过程的动态视图.
研究的目的:
- 开发一种快速,资源高效的基于转录基因组的衰老时钟,称为SkinAGE.
- 为了验证SkinAGE在不同条件下预测细胞衰老的准确性.
- 评估人类胚胎干细胞衍生的细胞外囊泡 (hESC-EVs) 在缓解细胞衰老方面的潜力.
主要方法:
- 在人类皮肤纤维细胞的基因表达特征上训练了一个深度神经网络 (DNN),以创建SkinAGE时钟.
- SkinAGE在独立的队列和UVB诱导的光衰老模型上进行了测试.
- 进行了转录基因分析,以调查衰老和青春复苏背后的分子机制.
主要成果:
- SkinAGE准确地预测了细胞衰老状态,UVB暴露显著增加了计算的年龄得分.
- 用hESC-EVs治疗有效降低了SkinAGE得分,表明转录基因衰老的逆转.
- 转录组数据显示,hESC-EV恢复了与衰老相关的基因表达模式,并调节了细胞周期进展和p53信号等关键信号通路.
结论:
- SkinAGE是一种可扩展,准确和具有成本效益的工具,用于量化细胞衰老.
- 这项研究表明hESC-EVs对细胞衰老的强大改善作用.
- SkinAGE提供了一个有价值的框架,用于评估新型复苏策略的有效性.
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