基于循环RNA的HPV16治疗疫苗产生强大而持久的抗瘤免疫力
Rong Zhou1, Chonghui Li2, Kunlun Xiang2
1Therorna Inc., Beijing, China.
Journal of experimental & clinical cancer research : CR
|January 8, 2026
概括
一种新型的循环RNA (circRNA) 疫苗有效向与人乳头瘤病毒 (HPV) 16相关的癌症. 这种脂质纳米颗粒封装的circRNA疫苗产生强烈的抗瘤免疫反应,导致瘤回归和增强免疫疗法的有效性.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
背景情况:
- 人类乳头瘤病毒 (HPV) 16感染与各种人类癌症有关.
- 治疗性疫苗为那些对标准治疗没有反应的患者提供了潜力.
- 循环RNA (circRNA) 为下一代癌症疫苗提供了一个稳定且可制造的平台.
研究的目的:
- 开发和评估一种编码融合HPV16 E7/E6抗原的合成circRNA疫苗.
- 在临床前模型中评估疫苗的免疫性和抗瘤反应.
- 研究circRNA疫苗与免疫检查点抑制剂的联合治疗.
主要方法:
- 开发了一种脂纳米粒子 (LNP) 封装的循环RNA疫苗 (LNP-circRNAE7E6) 编码融合的HPV16 E7/E6抗原.
- 在C57BL/6和TC-1瘤携带小鼠中评估了疫苗免疫性和抗瘤免疫反应.
- 通过流细胞计和免疫光学分析免疫细胞的变化;使用RNA测序检查基因表达和瘤微环境 (TME).
- 评估了与抗PD-L1抗体的联合治疗.
主要成果:
- 用LNP-circRNAE7E6进行首次增强疫苗接种诱导了显著的抗原特异性细胞毒性T细胞.
- 免疫治疗调节了TME,增加了免疫细胞透和促炎性基因表达.
- 通过LNP-circRNAE7E6疫苗接种,瘤完全回归,并防止瘤生长.
- 疫苗接种使瘤对免疫检查点阻塞疗法的敏感.
结论:
- LNP-circRNAE7E6疫苗在外周和瘤环境中产生强大的抗瘤免疫力.
- 这种基于circRNA的疫苗显示出治疗HPV16相关癌症的巨大潜力.
- 这些发现强调了预防癌症复发的治疗前景.
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