相关实验视频

Updated: Jun 23, 2026

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa

Published on: May 12, 2023

综合性植物化学和转录基因分析揭示了调节14-deoxyandrographolide的基因在泰国Andrographis paniculata

Neeranuch Thongkan¹, Worathat Thitikornpong², Amorntip Muangprom³

¹Center of Excellence in DNA Barcoding of Thai Medicinal Plants, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

|January 8, 2026

概括

这项研究研究了影响Andrographis paniculata14-deoxyandrographolide (AP6) 的遗传因素. 它确定了与AP6生物合成相关的候选基因,有助于标准化这种药用植物的质量.

科学领域:

植物化学植物化学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

安德罗格拉菲斯·帕尼库拉塔 (Andrographis paniculata) 是一种药用草本,其价值高于生物活跃的迪特尔类乳,如安德罗格拉菲利德 (AP1).
区域栽培导致植物化学特征的显著变化,影响原材料质量和治疗一致性.
14-deoxyandrographolide (AP6) 含量表现出显著的变化,这表明其积累的遗传基础.

研究的目的:

在40个泰国Andrographis paniculata的加入中全面描述diterpenoid乳和黄.
为了确定与对比AP6内容的加入,以便进一步调查.
通过比较转录组学获得对参与AP6生物合成的候选基因的初步见解.

主要方法:

使用HPTLC和HPLC进行植物化学分析,以量化二甲酸乳 (AP1,AP2,AP4,AP6).
通过UPGMA集群分析,将加入分为高AP6和低AP6组.
RNA测序 (RNA-seq) 用于在对比的AP6组之间识别差异表达基因 (DEG).

主要成果:

确定了四种主要的二类乳,其中AP1是最丰富的,AP6显示出最大的变异性.
进行比较的转录基因分析显示,高AP6和低AP6组之间有255个DEG.
候选基因,包括类似于CYP94C1,类似于CYP84A1和类似于beta-amyrin 28-monooxygenase的候选基因,被确定可能参与AP6生物合成.

结论:

这项研究整合了植物化学和转录基因数据,以探索Andrographis paniculata中AP6积累的遗传调节.
确定了AP6生物合成的新型候选基因,为未来的功能验证提供了基础.
这些发现支持植物化学标准化,精确的品种选择,以及从Andrographis paniculata开发具有附加值的草药产品.

更多相关视频

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Author Spotlight: Integrating 2D-HPLC-MS and Molecular Networking in Natural Medicine Analysis

Published on: December 8, 2023

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry

Published on: February 2, 2024

相关概念视频

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...