相关实验视频

Updated: Jan 13, 2026

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

6.5K

一个III型分泌系统效应器进化为机械不稳定,并从N端开始展开

Katherine E DaPron, Alexandre M Plastow, Morgan R Fink

    bioRxiv : the preprint server for biology
    |January 8, 2026
    PubMed
    概括

    细菌III型分泌系统 (T3SS) 需要蛋白质展开以分泌. 这项研究表明,细菌效应器在机械上是不稳定的,这是一个进化的特征,有助于T3SS展开和殖民.

    更多相关视频

    Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System09:12

    Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

    Published on: March 10, 2020

    7.6K
    Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification10:21

    Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification

    Published on: November 16, 2016

    13.9K

    相关概念视频

    Gram-negative Bacterial Protein Secretion Systems01:17

    Gram-negative Bacterial Protein Secretion Systems

    Gram-negative bacteria utilize sophisticated protein secretion systems to transport proteins across their double-membrane envelope into the extracellular environment or host cells. Based on their mechanism of action, these systems are classified into one-step and two-step pathways.One-Step Secretion Systems (Types I, III, IV, and VI)One-step secretion systems bypass the periplasm entirely, forming a continuous channel that spans both the inner and outer membranes:Type I Secretion System (T1SS):...
    753
    Bacterial Translocation and Protein Secretion01:26

    Bacterial Translocation and Protein Secretion

    Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
    505
    Insertion of Single-pass Transmembrane Proteins in the RER01:26

    Insertion of Single-pass Transmembrane Proteins in the RER

    Integral membrane proteins are proteins adhered to the lipid bilayer of a cell organelle or membrane. They can be of two types: transmembrane integral proteins that span the lipid bilayer and monotopic proteins that are attached to either side of the membrane but do not pass through it.
    Integral transmembrane proteins possess transmembrane and extra membrane domains. The transmembrane domains are primarily made of 20-25 hydrophobic amino acids arranged in a helical secondary confirmation. These...
    16.5K
    Bacterial Protein Maturation01:26

    Bacterial Protein Maturation

    Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
    447
    Protein Translocation Machinery on the ER Membrane01:28

    Protein Translocation Machinery on the ER Membrane

    The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
    Sec61 protein conducting channel
    In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
    6.5K
    Tail-anchoring of Proteins in the ER Membrane01:45

    Tail-anchoring of Proteins in the ER Membrane

    Tail-anchored, or TA, proteins are estimated to make up to 3-5% of membrane proteins found in the eukaryotic cell. Such proteins have a single transmembrane domain located approximately 30 amino acid residues upstream from the C-terminal end. As a result, the signal recognition particle (SRP) cannot guide a TA protein to the ER membrane for cotranslational insertion. Hence, they are integrated into the ER membrane post-translationally using their C-terminal end as the anchor. TA proteins...
    3.7K