超声导向的骨肌肉活检技术允许测量结构性,功能性,细胞性和生物化学性质
medRxiv : the preprint server for health sciences
|January 8, 2026
概括
超声波引导的真空辅助肌肉活检提供了充足的高质量组织,可从单次插入中进行详细分析. 这种微创手术提高了患者的舒适性,同时为组织学和功能研究提供了足够的样本.
科学领域:
- 生物医学工程 生物医学工程
- 肌肉骨研究 研究
- 最少侵入性手术的方法
背景情况:
- 传统的肌肉活检方法通常会产生不足的样本大小或损害组织质量.
- 细尺针活检在样本体积上存在限制,无法进行全面分析.
- 患者的不适是传统活检技术的一个重要问题.
研究的目的:
- 为了评估超声导向,真空辅助,单插入肌肉活检技术.
- 评估肌肉样本的产量,质量和适合性,用于各种分析.
- 确定新型活检方法的可行性和患者耐受性.
主要方法:
- 在19名健康参与者中,从一针插入广侧和前侧中收集了2-3个肌肉样本.
- 采用超声波引导和真空辅助吸收用于采样.
- 分析样品质量,尺寸,纤维截面积和量化的纤维.
主要成果:
- 从单次插入中获得的平均样本质量为148±38mg (VL) 和166±64mg (TA).
- 获得的样本适用于线粒体呼吸计,RT-PCR,原体分析和生物力学测试.
- 报告的低患者不适评级 (1.5/10立即,1.7/10在24小时后).
结论:
- 超声波引导的真空辅助肌肉活检产生了足够高质量的组织,用于广泛的组织学和生物化学分析.
- 这种单次插入技术尽量减少患者的不适和程序复杂性.
- 该方法耐受性良好,并为肌肉研究中的各种下游应用提供可行的样本.
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01:55Bone Structure
Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
01:17Gross Anatomy of Bone
The two main features of a long bone are the diaphysis and the epiphysis.
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in adults, it...
The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The walls of the diaphysis are composed of dense and hard compact bone made of numerous osteons — the functional unit of the compact bone. The hollow region in the diaphysis is called the medullary cavity, which harbors the bone marrow. In infants and children, this marrow cavity is filled with red marrow, whereas in adults, it...
01:09Spongy Bone
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...