在Drosophila melanogaster中,V-ATPase子单元M9.7-d对于精子运动至关重要
Si-Ying Li1, Hao-Lin Wang1, Qian Zhao1
1Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China.
在雄性果中击败VhaM9.7-d会通过损害精子运动和能量代谢而导致不育. 这突出了V-ATPase.
科学领域:
- 细胞生物学 细胞生物学
- 生殖生物学 生殖生物学
- 生物化学 生物化学
背景情况:
- 真空型质子ATPases (V-ATPases) 对细胞过程至关重要,但它们在生育中的作用尚不清楚.
- V-ATPases调节pH和离子运输,对器官功能和细胞能量至关重要.
研究的目的:
- 为了研究VhaM9.7-d的功能,一个V-ATPase子单元,在Drosophila melanogaster生育.
- 阐明VhaM9.7-d影响精子功能和男性生育能力的分子机制.
主要方法:
- 在雄性Drosophila生殖细胞中,VhaM9.7-d被淘汰.
- 评估男性和女性的生育能力,精子运动能力和受精率.
- 双重质量标签 (TMT) 是精子囊泡 (SV) 的蛋白质组.
- 发酵体和线粒体功能的测定 (LysoSensor,CMXRos,ATP测量).
主要成果:
- 在不影响女性生育能力的情况下,VhaM9.7-d knockdown诱导了完全的男性不育.
- 来自VhaM9.7-d贫乏男性的精子失去了运动能力,无法受精卵.
- 蛋白质组分析揭示了蛋白质表达的改变,特别是在细胞和氧化酸化 (OxPHOS) 途径中.
- VhaM9.7-d敲击破坏了SV酸度,增加了活性氧物种 (ROS),并降低了线粒体膜潜力和ATP水平.
结论:
- VhaM9.7-d对于维持精子能量代谢在雄性Drosophila. homeostasis中至关重要.
- 它调节了法戈利索马活动和线粒体OxPHOS,对精子运动和受精能力至关重要.
- 这些发现提供了对人类精子症机制的见解.
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