通过Cupriavidus necator KCTC 2649使用子 (Cocos nucifera L.) 水作为碳源,生物合成聚酸 (PHA)
Jaren U Tulipan1, John Rafael C Unlayao1, Gewelle Mae L Punzalan1
1Biomaterials and Environmental Engineering Laboratory, Department of Engineering Science, College of Engineering and Agro-Industrial Technology, University of the Philippines Los Baños, Laguna, Philippines.
Preparative biochemistry & biotechnology
|January 8, 2026
概括
子水有效地支持Cupriavidus necator的多氧酸盐 (PHA) 生产. 过器灭菌和优化的C/N和C/P比率显著提高了生物质和PHA产量,展示了可持续的生物聚合物来源.
科学领域:
- 生物技术是生物技术.
- 微生物生产 微生物生产
- 可持续的高分子.
背景情况:
- 聚酸酸盐 (PHA) 是微生物合成的可生物降解聚合物.
- 子水是一种丰富的,具有成本效益的,富含碳水化合物的农业工业副产品.
- 目前的PHA生产通常依赖于昂贵且不可持续的碳来源.
研究的目的:
- 调查介质灭菌方法对使用子水生产PHA产生的影响.
- 优化碳与 (C/N) 和碳与 (C/P) 的比率,以提高PHA产量.
- 评估子水作为由Cupriavidus necator进行PHA生物合成的可持续基质.
主要方法:
- 测试各种介质灭菌技术 (新鲜的,自,过器灭菌的,合成的).
- 使用子水作为碳源种植Cupriavidus necator KCTC 2649.
- 系统地改变C/N和C/P比率以确定PHA生产的最佳条件.
主要成果:
- 过器消毒的介质产生了4.31g/L (0.431g/g糖) 的最高PHA.
- 最佳的营养比 (C/N为50和C/P为10) 最大化了生物质和PHA生产 (5.85 g/L;59.57%重量/重量).
- 在高C/N和C/P比率下限制营养素被发现可以促进PHA积累.
结论:
- 子水是微生物PHA生产的可行和可持续基质.
- 介质灭菌和精确的营养比控制对于最大限度地提高PHA产量至关重要.
- 库普里亚维杜斯 (Cupriavidus necator) 有效地利用子水用于生物质和聚酸酸合成.
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