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通过全面的二维气体染色学,飞行时间质谱学和图像图案识别来推进便挥发体的概况

Fulvia Trapani¹, Andrea Caratti¹, Erica Liberto¹

¹Dipartimento Di Scienza E Tecnologia del Farmaco, Università Di Torino, Via Giuria 9, 10125, Turin, Italy.

Analytical and bioanalytical chemistry

|January 8, 2026

概括

这项研究使用先进的GC×GC-TOFMS来分析非乳性质敏感患者的肠道细菌. 益生菌使用改变了便的挥发性有机化合物,显示了个性化营养策略的潜力.

科学领域:

分析化学分析化学
代谢学代谢学代谢学
微生物组研究微生物组研究

背景情况:

非乳性质敏感性 (NCGWS) 涉及对质的复杂肠道反应.
了解便挥发性瘤对于诊断和管理NCGWS至关重要.
饮食干预和益生菌可以调节肠道微生物群和代谢物生产.

研究的目的:

在接受饮食干预的NCGWS患者中描述便挥发性有机化合物 (VOC) 概况.
研究重新引入质和益生菌补充剂对便代谢物的影响.
建立GC×GC-TOFMS和染色指纹作为个性化营养中的生物标志物发现工具.

主要方法:

使用了全面的二维气体染色学-飞行时间质谱法 (GC×GC-TOFMS).
头部空间固态微提取 (HS-SPME) 被优化为挥发性提取.
使用了组合的非定向-定向 (UT) 指纹和化学分析 (PCA,PLS-DA).

主要成果:

绘制了大约1000个挥发性特征,注释了约270个VOC,将便挥发性物质的知识扩大了20%.
由于重新引入质和给予益生菌,观察到VOC概况的显著差异.
PLS-DA模型准确地区分了接受益生菌治疗的受试者 (准确率为89-90%),表明益生菌对肠道代谢特征的影响.

结论:

结合基于图像的色谱指纹,GC×GC-TOFMS对分析复杂的便代谢组有效.
益生菌补充可以证明改变NCGWS患者的肠道代谢概况.
这种综合方法显示出在饮食干预和个性化营养中发现生物标志物的前景.

关键词:

综合性的二维气体染色学.便的挥发性物质学图像模式识别图像模式识别非乳性质敏感性的人.UT指纹的使用方法

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