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慢性压力通过降低聚焦粘附激酶相关蛋白质复合物的调节,破坏了老鼠丸中的外质体内特异化动力

Itishree Dubey¹, Sapana Kushwaha²

¹Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER-R), Raebareli, Lucknow, (UP), 226002, India.

Reproductive sciences (Thousand Oaks, Calif.)

|January 8, 2026

概括

长期的压力会通过破坏血液-丸屏障和损害精子生产,逐渐损害男性生殖健康. 长时间的压力暴露显著降低了精子质量和荷尔蒙水平,导致男性不孕.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
压力生理学压力生理学

背景情况:

慢性压力对男性生殖健康产生负面影响,导致全球不孕症.
血液-丸屏障 (BTB) 对于精子生成至关重要,容易受到压力诱导的破坏.
BTB完整性的关键调节者,包括β1-整合素和焦粘附激酶 (FAK) 信号,在长期应激模型中涉及但尚未完全理解.

研究的目的:

研究慢性不可预测压力 (CUS) 对Sprague-Dawley大鼠雄性生殖功能的长期影响.
阐明CUS诱导的丸损伤背后的分子机制,重点关注BTB完整性和精子生成.
评估压力持续时间对生殖功能障碍严重性的影响.

主要方法:

鼠被暴露在CUS中的时间为8,14或18周.
评估行为变化,荷尔蒙概况 (FSH,LH,) 和精子参数 (数量,运动性,形态,染色质完整性).
利用西方斑点来分析BTB标记物,细胞亡和关键信号通路 (FAK,Akt,mTOR).

主要成果:

CUS引发了抑郁和焦虑类行为,皮质水平升高,探索活动减少.
观察到精子数量,运动性,形态和染色质完整性的显著减少,随着压力持续时间 (14周和18周) 恶化.
发现FSH,LH,丸激素,BTB标志物,11β-HSD和虹膜炎的降低,以及丸亡的增加和FAK/Akt/mTOR信号通路的破坏.

结论:

长期的CUS对男性生殖功能产生累积的不良影响,严重程度取决于压力的大小和持续时间.
通过破坏特定的分子信号干扰,CUS破坏了BTB的完整性,破坏了ectoplasmic专业化的稳定性,并松了紧密的结节.
这些发现凸显了压力诱导的生殖功能障碍及其潜在机制的渐进性,影响精子生成.

关键词:

BTBTB BTBTB 在线观看慢性不可预测的压力.细胞外质的专业化.焦点粘附激酶的焦点粘附激酶男人生殖系统男人生殖系统测试 (Testis) 是一个测试.

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