糖基-tRNA合成酶与孟科病毒RNA的结合刺激了翻译
Fabian Droß1, Tim Gödert1, Selena A Fuchshuber1
1Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, 35392 Giessen, Germany.
Nucleic acids research
|January 8, 2026
概括
孟哥病毒RNA利用tRNA抗干环状结构来结合细胞甘氨酸-tRNA合成酶 (GARS),增强病毒翻译和复制. 一个拟议的GARS二极管模型解释了5'和3'未翻译区域之间的这种相互作用.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 皮科纳病毒依赖于与细胞蛋白相互作用的RNA二级结构来进行翻译和复制.
- 孟戈病毒是一种心脏病毒,在其未翻译区域 (UTR) 中具有独特的RNA元素.
研究的目的:
- 为了表征tRNA抗子干环状结构在孟科病毒RNA.
- 研究这些结构与细胞蛋白质的结合,特别是糖基-tRNA合成酶 (GARS).
- 阐明这些相互作用在病毒翻译和复制中的作用.
主要方法:
- 在GARS结合元件 (GBE) 和GARS.中保存的基因的位点定向突变发生.
- 报告者RNA测试以评估翻译效率.
- 转化因子和核糖体招募的分析.
主要成果:
- 孟戈病毒5和3UTR含有具有tRNA抗根茎循环状结构的GARS结合元件 (GBEs).
- 证实了GARS与GBE的特定结合;突变破坏了这种受损的结合.
- 3 -UTR GBE的突变减少了早期的病毒翻译,而不依赖于聚合酶活性.
- 3 -UTR GBE是一个更强的GARS结合部位,显著招募翻译因子和核糖体.
- 5 -UTR GBE虽然是一种较弱的结合部位,但对突变后的翻译有更明显的影响.
结论:
- 提出了一个GARS二元模型,其中一个单体在3-UTR GBE上,另一个与5-UTR GBE相互作用.
- 这种相互作用刺激翻译因子和核糖体招募,增强孟戈病毒的翻译.
- RNA-GARS相互作用对于宿主细胞内的有效病毒复制至关重要.
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