通过组织蛋白甲基化和小RNAs协调的效应基因沉默增强了植物病原体中的宿主适应
Liyuan Wang1,2, Xuewei Xiang1, Guoyu Yin2
1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Nucleic acids research
|January 8, 2026
概括
病原体的适应性依赖于通过基因组三甲基化 (H3K27me3) 和小RNAs (sRNAs) 的协调表观遗传沉默. 这项研究揭示了PsSu(z) 12在Phytophthora sojae中协调这些免疫逃避机制.
科学领域:
- 植物病理学 植物病理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 分子微生物学 分子微生物学
背景情况:
- 病原体通过表观遗传机制的适应性,特别是素3素27三甲基化 (H3K27me3) 和小RNA (sRNA) 介导的基因沉默,对于免疫逃避至关重要,但人们对其了解甚少.
- 像Phytophthora sojae这样的Oomycete病原体利用激烈性 (Avr) 基因来逃避宿主抗性 (R) 蛋白质,这对疾病管理构成了挑战.
研究的目的:
- 研究H3K27me3和sRNA介导的沉默在调节Phytophthora sojae中的Avr基因中的协调作用.
- 确定关键的表观遗传调节者,参与病原体适应性和免疫逃避.
主要方法:
- 在CRISPR/Cas9中介的PsSu(z) 12基因编辑,这是Polycomb抑制复合体2 (PRC2) 的组成部分.
- 对于H3K27me3,RNA测序和sRNA测序的染色质免疫沉 (ChIP).
- 在田间分离的表观遗传变异的分析.
主要成果:
- 破坏PsSu(z) 12取消了H3K27me3沉积,重新激活了Avr基因,并导致激发性丧失.
- H3K27me3和sRNAs在效应基因中显示出特定位置的共同丰富和共同沉默模式.
- 在现场隔离物中观察到表观遗传变异,表明效应基因调节中的可塑性.
结论:
- PsSu(z) 12作为一个中央调节器,协调H3K27me3和sRNA介导的Phytophthora sojae中Avr基因的沉默.
- 涉及H3K27me3和sRNA的双层表观遗传机制使病原体能够逃避免疫和适应.
- 了解这些表观遗传过程对于开发可持续的疾病管理策略至关重要.
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