优化的共同发酵策略,以使用工程Escherichia coli和Saccharomyces cerevisiae来有效地从β-Ionol中生产Dihydro-β-ionone
Kaixuan Ke1, Jiafeng Li1, Huilin Xie1
1State Key Laboratory for the Development and Utilization of Forest Food Resources, Nanjing Forestry University, Nanjing 210037, China; College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Bioorganic chemistry
|January 8, 2026
概括
这项研究开发了一个共同培养系统,使用大肠杆菌和大肠杆菌来生物合成二-β-ionone. 优化的两步微生物工艺实现了这种有价值的香料化合物的高产量.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 代谢工程是代谢工程.
背景情况:
- 二-β-离子是一种高价值的挥发性化合物,在香水,化品和食品中具有应用.
- 对二-β-离子子的可持续生物合成越来越感兴趣.
- 现有的生产方法可能不是高效或可持续的.
研究的目的:
- 开发和优化一个模块化共同培养系统,用于从β-ionol中生物合成二-β-ionone.
- 为了提高二β-离子子生产的效率和产量.
- 为工业生物技术应用提供技术支持.
主要方法:
- 通过使用大肠杆菌和麦片菌菌 (Saccharomyces cerevisiae) 建立了一个模块化共培系统.
- 大肠杆菌表达了NaSDR和葡萄糖脱酶 (GDH) 以β-离子醇氧化为β-离子子.
- 在S. cerevisiae表达的双键还原酶AadBR1用于β-离子体减少为二-β-离子体,与Pos5p用于NADPH再生.
主要成果:
- 大肠杆菌模块实现了884.62 mg/L的β-离子和44.2%的转换效率.
- 在S. cerevisiae模块中,产生了829.71 mg/L的二-β-离子子,转换效率为41.45%.
- 通过在两个微生物宿主中脱氧化和还原步骤来实现最佳性能.
结论:
- 开发的共同培养系统使得有效的微生物生产二-β-离子.
- 在不同的微生物宿主中脱生物合成步骤提高了生产效率.
- 这种方法为工业规模生产二-β-离子子和其衍生物提供了潜力.
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