含有挥发性脂肪酸的合成气发酵废水对聚酸酸酸盐生产的影响
Nirpesh Dhakal1, Darren Korber2, Sudarshana Bhumireddy3
1Department of Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK S7N 5A9, Canada.
Bioresource technology
|January 8, 2026
概括
这项研究将合成气发酵与大麻粉水解物结合起来,以产生挥发性脂肪酸 (VFA) 和多基酸盐 (PHA). 这两阶段的工艺有效地利用农业副产品将废物流转化为有价值的生物聚合物.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 可持续化学 可持续化学
- 微生物发酵 微生物发酵
背景情况:
- 合成气发酵是将富含碳的废物转化为有价值的化学品的关键技术.
- 挥发性脂肪酸 (VFA) 是生产生物聚合物,如聚酸酸盐 (PHA) 的关键中间体.
- 利用低价值的农业副产品作为营养来源可以提高生物工艺的经济可行性.
研究的目的:
- 评估一种新的两阶段发酵策略,用于从合成气中生产VFA,随后合成PHA.
- 评估作为营养补充剂的甘油粉衍生蛋白质水解盐 (CanXtract,CX) 的有效性.
- 利用合成气和农业废物优化VFA生产和PHA合成的条件.
主要方法:
- 采用了两阶段的轴性发酵过程,使用*Clostridium carboxidivorans*用于VFA生产和*Cupriavidus necator*用于PHA合成.
- 合成气发酵是在不同的CanXtract度和CO部分压力下进行的.
- 使用基于VFA成分的中央复合设计优化了PHA生产,并补充了营养素.
主要成果:
- 与酵母提取物相比,CanXtract显著增强了微生物生长和VFA生产,最佳度≥75%.
- 最大VFA生产率达到0.71g/L/d,主要是酸和黄油酸.
- 最佳的PHA合成发生在5g/L的酸和2.5g/L的黄油酸中,通过补充达到76%的PHA含量和1.11g/L/d的生产率.
结论:
- 集成的两阶段发酵平台有效地将合成气和甘露粉副产品提升为PHA.
- CanXtract作为一个具有成本效益的营养来源,改善VFA和PHA产量.
- 这种方法证明了从废物流中生产生物聚合物的可持续和经济可行的途径.
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