核桃蛋白-多糖化合物异质复合物的结构和机制分析:提高溶解性和功能性质
Weiye Jiang1, Mingxin Zhang2, Liang Zhao1
1Key Laboratory of Geriatric Nutrition and Health, Beijing Technology and Business University, Ministry of Education, Beijing, 100048, PR China.
International journal of biological macromolecules
|January 8, 2026
概括
通过pH循环辅助的fucoidan结合显著增强了核桃蛋白分离物 (WPI) 的功能. 这种方法重组了WPI,提高了其乳化能力和界面稳定性,以获得更好的食品应用.
科学领域:
- 食品科学与技术 食品科学与技术
- 生物材料科学 生物材料科学
- 蛋白质化学 蛋白质化学
背景情况:
- 植物蛋白分离物,如核桃蛋白分离物 (WPI),具有功能限制.
- 多糖合是一种提高蛋白质功能的一种策略.
- 富科伊丹是蛋白质修饰的一个有前途的多糖.
研究的目的:
- 为了研究pH循环介导的异质结合与富可伊丹对WPI的影响.
- 为了确定功能增强的最佳WPI:fucoidan比率.
- 阐明结合过程引起的结构变化.
主要方法:
- 对五种多糖的系统选.
- 使用pH循环,将WPI与富可伊丹结合在一起.
- 多光谱分析 (SDS-PAGE,CD,光,UV-Vis) 进行.
- 物理化学特征 (粒子大小,表面水性,泽塔潜力).
主要成果:
- 福科伊丹被确定为最佳的配偶伴侣,WPI:福科伊丹比率为1:0.7.7.
- 结合引发了显著的结构变化:α螺旋减小,β片含量增加,三级结构改变.
- 物理化学性质得到了修改:粒子大小增加,表面水性降低,以及更多的负泽塔潜力.
- 观察到增强的乳化能力和界面稳定性.
结论:
- 通过pH循环辅助的fucoidan结合是改善WPI功能的一种有效策略.
- 控制的结构重组是提高植物蛋白性能的关键.
- 这种方法为开发改进的植物成分提供了潜力.
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