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阿斯特拉加勒斯多糖化物通过促进AMPK酸化来改善冷藏后的狗精子质量

Xiaogang Huang¹, Zhihong Zhao², Ronggen Wang²

¹State Key Laboratory of Swine and Poultry Breeding Industry, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agroanimal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.

BMC veterinary research

|January 8, 2026

概括

阿斯特拉加勒斯多糖 (APS) 通过提高运动性和活力来增强狗精子的保存. 它通过促进AMPK酸化来防止氧化损伤,为犬精液储存提供了一种新的策略.

科学领域:

兽医科学兽医科学兽医科学
生殖生物学生殖生物学
生物化学生物化学

背景情况:

实验室精液保存对于保护犬的遗传物质至关重要.
储存期间的氧化应激会显著降低精子的活力和功能.
外源抗氧化剂对于提高体外精液储存效率至关重要.

研究的目的:

为了研究Astragalus多糖 (APS) 在冷藏后对狗精子质量的影响.
阐明APS在保护狗精子中的潜在机制.

主要方法:

狗精液被冷却并存储在不同度的APS中.
评估了精子参数,包括运动性,等离子体膜完整性,体完整性和线粒体膜潜力.
测量了精子的抗氧化能力和脂质过氧化水平.
评估了AMPK酸化和AMPK抑制剂 (化合物C) 的作用.

主要成果:

0.50 mg/mL的APS补充剂显著改善了精子运动性,血膜和体完整性以及线粒体膜潜力.
APS增强了精子的抗氧化能力,降低了脂质过氧化.
APS促进了AMPK的酸化,而这种效应被AMPK抑制剂部分阻断,这表明AMPK的关键作用.

结论:

阿斯特拉加勒斯多糖 (APS) 在冷藏期间有效地保护狗精子.
通过促进AMPK酸化,APS增强了精子的抗氧化能力和活力.
APS是狗精子扩展器的可行补充,为精子保存提供了一种新的方法.

关键词:

阿斯特拉加勒斯的多糖化物 (APS)AMPK的酸化方式狗的精子是狗的精子冷藏储存的冷藏方式

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